ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2018
Zebrafish Rfx4 controls dorsal and ventral midline formation in the neural tube.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 28 citations in OpenAlex.
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- A genomic compendium of hundreds of teleost fishes reveals their evolutionary landscape.Innovation (Cambridge (Mass.)) · 2026Article
- A Human Neural Tube Model Using 4D Self-Folding Smart Scaffolds.Advanced healthcare materials · 2026Article
- The invasion phenotypes of glioblastoma depend on plastic and reprogrammable cell states.Nature communications · 2025Article
- Medulloblastoma's master regulators and their association with patients' risk.Scientific reports · 2025Article
- Pediatric Diffuse Midline Glioma H3K27-Altered: From Developmental Origins to Therapeutic Challenges.Cancers · 2024Review
- RFX4 is an intrinsic factor for neuronal differentiation through induction of proneural genes POU3F2 and NEUROD1.Cellular and molecular life sciences : CMLS · 2024Article
- An RFX transcription factor regulates ciliogenesis in the closest living relatives of animals.Current biology : CB · 2023Article
- scChIX-seq infers dynamic relationships between histone modifications in single cells.Nature biotechnology · 2023Article
- The Intricate Epigenetic and Transcriptional Alterations in Pediatric High-Grade Gliomas: Targeting the Crosstalk as the Oncogenic Achilles' Heel.Biomedicines · 2022Review
- Organoids as a new model system to study neural tube defects.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Review
- Article
- Derivedness Index for Estimating Degree of Phenotypic Evolution of Embryos: A Study of Comparative Transcriptomic Analyses of Chordates and Echinoderms.Frontiers in cell and developmental biology · 2021Article
- Gene Environment Interactions in the Etiology of Neural Tube Defects.Frontiers in genetics · 2021Review
- Interplay of RFX transcription factors 1, 2 and 3 in motile ciliogenesis.Nucleic acids research · 2020Article
- Modeling Neuronal Diseases in Zebrafish in the Era of CRISPR.Current neuropharmacology · 2020Review
- All-age whole mount in situ hybridization to reveal larval and juvenile expression patterns in zebrafish.PloS one · 2020Article
- Histone Variant and Cell Context Determine H3K27M Reprogramming of the Enhancer Landscape and Oncogenic State.Molecular cell · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundRfx winged-helix transcription factors, best known as key regulators of core ciliogenesis, also play ciliogenesis-independent roles during neural development. Mammalian Rfx4 controls neural tube morphogenesis via both mechanisms.
resultsWe set out to identify conserved aspects of rfx4 gene function during vertebrate development and to establish a new genetic model in which to analyze these mechanisms further. To this end, we have generated frame-shift alleles in the zebrafish rfx4 locus using CRISPR/Cas9 mutagenesis. Using RNAseq-based transcriptome analysis, in situ hybridization and immunostaining we identified a requirement for zebrafish rfx4 in the forming midlines of the caudal neural tube. These functions are mediated, least in part, through transcriptional regulation of several zic genes in the dorsal hindbrain and of foxa2 in the ventral hindbrain and spinal cord (floor plate).
conclusionsThe midline patterning functions of rfx4 are conserved, because rfx4 regulates transcription of foxa2 and zic2 in zebrafish and in mouse. In contrast, zebrafish rfx4 function is dispensable for forebrain morphogenesis, while mouse rfx4 is required for normal formation of forebrain ventricles in a ciliogenesis-dependent manner. Collectively, this report identifies conserved aspects of rfx4 function and establishes a robust new genetic model for in-depth dissection of these mechanisms. Developmental Dynamics 247:650-659, 2018. © 2017 Wiley Periodicals, Inc.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.