ArticleOdontology2018
Effect of root canal sealers on human periodontal ligament fibroblast viability: ex vivo study.
Article in Odontology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Physicochemical Characterization and In Vitro Biocompatibility of Epoxy- and Silicone-Based Endodontic Materials.Materials (Basel, Switzerland) · 2026Article
- Cytotoxicity of Root Canal Sealers and Potential Clinical Implications: A Comprehensive Systematic Review of In Vitro Studies.Journal of clinical medicine · 2026Review
- In Vitro and In Vivo Evaluation of a New Experimental Polydimethylsiloxane-Based Endodontic Sealer.Journal of functional biomaterials · 2025Article
- Enhancing the Biological Properties of Organic-Inorganic Hybrid Calcium Silicate Cements: An In Vitro Study.Journal of functional biomaterials · 2024Article
- Impact of various endodontic sealers on HPDLF Cell viability and apoptosis.Scientific reports · 2024Article
- Cytotoxicity and genotoxicity of bioceramic root canal sealers compared to conventional resin-based sealer.Scientific reports · 2024Article
- Effect of Different Sealers on the Cytocompatibility and Osteogenic Potential of Human Periodontal Ligament Stem Cells: An In Vitro Study.Journal of clinical medicine · 2023Article
- A comprehensive in vitro comparison of the biological and physicochemical properties of bioactive root canal sealers.Clinical oral investigations · 2022Article
- The Assessment of Quality of the Root Canal Filling and the Number of Visits Needed for Completing Primary Root Canal Treatment by Operators with Different Experience.Bioengineering (Basel, Switzerland) · 2022Article
- Article
- Influence of Commonly Used Endodontic Irrigants on the Setting Time and Metal Composition of Various Base Endodontic Sealers.Polymers · 2021Article
- The Structural, Physical, and In Vitro Biological Performance of Freshly Mixed and Set Endodontic Sealers.European endodontic journal · 2021Article
- Pleiotropic Effects of Eugenol: The Good, the Bad, and the Unknown.Oxidative medicine and cellular longevity · 2021Review
- Physical Properties, Antimicrobial Activity and In Vivo Tissue Response to Apexit Plus.Materials (Basel, Switzerland) · 2020Article
- Biocompatibility of Root Canal Sealers: A Systematic Review of In Vitro and In Vivo Studies.Materials (Basel, Switzerland) · 2019Review
- Evaluation of the biocompatibility of root canal sealers on human periodontal ligament cells ex vivo.Odontology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The aim of the study was to compare ex vivo the toxic effects of six root canal sealers immediately after mixing or setting on human periodontal ligament fibroblasts (HPdLF). Freshly mixed (I group) or set (allowed to dry for 24 h) (II group) specimens of AH Plus Jet (AH), Apexit Plus (AP), MTA Fillapex (FL), GuttaFlow (GF), MetaSEAL Soft (META), and Tubli-Seal (TS) were prepared. HPdLF were exposed for 24 h to the specimens. 3-(4,5-dimethylthiazolo-2-yl)-2,5-diphenyltetrazolium bromide assay was used to examine the effect of the root canal sealers on mitochondrial metabolic activity. Fluorescein isothiocyanate (FITC)-annexin V (AnV) and propidium iodide staining followed by flow cytometry was used to identify the effects of the materials on cell apoptosis/necrosis. Statistical analyses were performed by one-way ANOVA followed by post hoc tests, and significance was determined at P < 0.05. Most materials from the two groups reduced the viability of the cultured cells compared with the control group (P < 0.05). Statistical analysis showed significant differences in HPdLF viability between the individual materials in each group (P < 0.001). AH and AP induced a significant increase in the percentage of apoptotic cells, while TS, FL, and META elevated the proportion of necrotic cells compared with other materials and the controls (p < 0.05). The cytotoxic effects of the tested root canal sealers (both fresh and set) on HPdLF varied. Both forms of sealers were able to cause toxic effects by inducing apoptosis and necrosis in HPdLF. The cytotoxicity of FL, META, TS was mainly associated with necrosis, while AH and AP with apoptosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.