Evidence map›Paper›PMID 29242709›Full record

ArticleNorth American journal of aquaculture2017

Standardized Assessment of Thin-film Vitrification for Aquatic Species.

Nolan J Tiersch, Terrence R Tiersch

Abstract read
In one paragraph

Article in North American journal of aquaculture, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Nolan J TierschAquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, 2288 Gourrier Avenue, Baton Rouge, Louisiana 70820, USA.
Terrence R TierschAquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, 2288 Gourrier Avenue, Baton Rouge, Louisiana 70820, USA.

Funding

Enhanced Development of the Xiphophorus Model System R24-OD-011120R24OD011120 · OD · TEXAS STATE UNIVERSITY · PI WALTER, RONALD B · 2012 to 2019
$5.3M
Development of Germplasm Resources for Preservation of Aquatic ModelsR24OD010441 · OD · LOUISIANA STATE UNIV AGRICULTURAL CENTER · PI BLACKBURN, HARVEY D., MONROE, WILLIAM · 2012 to 2021
$4.3M
Development of Germplasm Resources for Preservation of Aquatic ModelsR24RR023998 · NCRR · LOUISIANA STATE UNIV AGRICULTURAL CENTER · PI TIERSCH, TERRENCE ROBERT · 2008 to 2011
$2.3M
NCRR NIH HHS R24 RR023998NIH HHS R24 OD010441NIH HHS R24 OD011120
6 · The paper itself

Abstract

Ultra-rapid cooling under the appropriate conditions will produce vitrification, a glass-like state used to cryopreserve small sample volumes, but there are a number of major technical drawbacks impeding application of vitrification to germplasm of aquatic species. These include a lack of suitable devices, and poor reproducibility and comparability among studies due to a lack of standardization. We used 3-dimensional (3-D) printing to produce a viewing pedestal coupled with a classification system to rapidly assess frozen film quality of vitrification loops. Classification time declined with practice from 2.1 ± 0.3 sec to 1.5 ± 0.2 sec (after 200 assessments), and assessments were consistently made in < 2.5 sec. Classifications should be reported with representative images allowing harmonization for quality control. This approach permits rapid classification and can be applied for development of methods including evaluation of vitrification solution components, concentrations of solution and target cells, and configurations and volumes of new devices. Future studies should address the custom fabrication of 3-D printed vitrification devices for use with aquatic species and other applications.

Identifiers

PMID29242709
PMCPMC5724558

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.