ArticleNature communications2017
Efficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Heritable transgenic schistosomes as a living platform for SARS-CoV-2 neutralizing antibody secretion.Nature communications · 2026Article
- Article
- Curation of the Fasciola hepatica kinome as a resource for drug target discovery.BMC genomics · 2026Article
- Tracking karyotype dynamics by flow cytometry reveals de novo chromosome duplications in laboratory cultures of Macrostomum lignano.Biology open · 2026Article
- Heat-induced transcriptional activation of LTR retrotransposons in the regenerative flatworm Macrostomum lignano.Mobile DNA · 2025Article
- Knockdown-Induced Fasting Phenotypes in Flatworms: Insights into Underlying Mechanisms of Feeding Behavior.International journal of molecular sciences · 2025Article
- One probe fits all: a highly customizable modular RNA in situ hybridization platform expanding the application of SABER DNA probes.Development (Cambridge, England) · 2025Article
- The Early Evolution of Tudor Genes in Holozoa and How Their Distribution Was Influenced by Life History Traits in Metazoa.Genome biology and evolution · 2025Article
- Review
- A genetic and microscopy toolkit for manipulating and monitoring regeneration in Macrostomum lignano.Cell reports · 2024Article
- Transgenesis enables mapping of segmental ganglia in the leech Helobdella austinensis.The Journal of experimental biology · 2024Article
- Arthropod promoters for genetic control of disease vectors.Trends in parasitology · 2024Review
- Optimized protocols for RNA interference in Macrostomum lignano.G3 (Bethesda, Md.) · 2024Article
- Sex-limited experimental evolution drives transcriptomic divergence in a hermaphrodite.Genome biology and evolution · 2024Article
- Diatoms-endoparasite association in fish from the marine pacific coast of Colombia (Buenaventura).PloS one · 2024Article
- Unraveling the Unusual Subgenomic Organization in the Neopolyploid Free-Living Flatworm Macrostomum lignano.Molecular biology and evolution · 2023Article
- Genome assemblies of the simultaneously hermaphroditic flatworms Macrostomum cliftonense and Macrostomum hystrix.G3 (Bethesda, Md.) · 2023Article
- The microbiome of the marine flatworm Macrostomum lignano provides fitness advantages and exhibits circadian rhythmicity.Communications biology · 2023Article
- Pluripotency retention and exogenous mRNA introduction in planarian stem cells in culture.iScience · 2023Article
- Genome assembly of the acoel flatworm Symsagittifera roscoffensis, a model for research on body plan evolution and photosymbiosis.G3 (Bethesda, Md.) · 2023Article
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Authors and funding
20 authors.
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Abstract
Regeneration-capable flatworms are informative research models to study the mechanisms of stem cell regulation, regeneration, and tissue patterning. However, the lack of transgenesis methods considerably hampers their wider use. Here we report development of a transgenesis method for Macrostomum lignano, a basal flatworm with excellent regeneration capacity. We demonstrate that microinjection of DNA constructs into fertilized one-cell stage eggs, followed by a low dose of irradiation, frequently results in random integration of the transgene in the genome and its stable transmission through the germline. To facilitate selection of promoter regions for transgenic reporters, we assembled and annotated the M. lignano genome, including genome-wide mapping of transcription start regions, and show its utility by generating multiple stable transgenic lines expressing fluorescent proteins under several tissue-specific promoters. The reported transgenesis method and annotated genome sequence will permit sophisticated genetic studies on stem cells and regeneration using M. lignano as a model organism.
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