Evidence map›Paper›PMID 29241586›Full record

ArticleCellular immunology2018

Influence of membrane cholesterol on monocyte chemotaxis.

Amit K Saha, Marzieh Mousavi, Shatha F Dallo, Shankar J Evani, Anand K Ramasubramanian

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular immunology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. New polyoxazoline loaded patches for hemostasis in experimental liver resection.Journal of biomedical materials research. Part B, Applied biomaterials · 2022
    Article
  4. Review
  5. Cyclodextrins reduce the ability of Pseudomonas aeruginosa outer-membrane vesicles to reduce CFTR ClAmerican journal of physiology. Lung cellular and molecular physiology · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Amit K SahaDepartment of Biomedical, Chemical and Materials Engineering, San José State University, San José, CA 95192, USA.
Marzieh MousaviDepartment of Biomedical, Chemical and Materials Engineering, San José State University, San José, CA 95192, USA.
Shatha F DalloDepartment of Biomedical Engineering, The University of Texas at San Antonio, San Antonio, TX 78249, USA.
Shankar J EvaniDepartment of Biomedical Engineering, The University of Texas at San Antonio, San Antonio, TX 78249, USA.
Anand K RamasubramanianDepartment of Biomedical, Chemical and Materials Engineering, San José State University, San José, CA 95192, USA. Electronic address: anand.ramasubramanian@sjsu.edu.
San Jose State University · USThe University of Texas at San Antonio · US

Funding

RCMI Center for Interdiscplinary Health Research CIHRG12MD007591 · NIMHD · UNIVERSITY OF TEXAS SAN ANTONIO · PI PERRY, GEORGE · 2012 to 2016
$10.4M
Role of fluid flow on Chlamydia pneumoniae-exacerbated atherosclerosisSC1HL112629 · NHLBI · UNIVERSITY OF TEXAS SAN ANTONIO · PI RAMASUBRAMANIAN, ANAND K · 2011 to 2015
$1.8M
NHLBI NIH HHS SC1 HL112629NIMHD NIH HHS G12 MD007591
6 · The paper itself

Abstract

Cholesterol content influences several important physiological functions due to its effect on membrane receptors. In this work, we tested the hypothesis that cellular cholesterol alters chemotactic response of monocytes to Monocyte Chemoattractant Protein-1 (MCP-1) due to their effect on the receptor, CCR2. We used Methyl-β-cyclodextrin (MβCD) to alter the baseline cholesterol in human monocytic cell line THP-1, and evaluated their chemotactic response to MCP-1. Compared to untreated cells, cholesterol enrichment increased the number of monocytes transmigrated in response to MCP-1 while depletion had opposite effect. Using imaging flow cytometry, we established that these differences were due to alterations in expression levels, but not the surface distribution, of CCR2.

Indexed as

beta-CyclodextrinsCells, CulturedChemokine CCL2Chemotaxis, LeukocyteCholesterolHumansMonocytesReceptors, CCR2Receptors, ChemokineTHP-1 Cellsbeta-CyclodextrinsCCL2 protein, humanCCR2 protein, humanChemokine CCL2Cholesterolmethyl-beta-cyclodextrinReceptors, CCR2Receptors, ChemokineAMNISCCR2CholesterolMCP-1MβCDTHP-1Transwell

Identifiers

PMID29241586
PMCPMC5808849
OpenAlexW2772836791

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.