ArticleCell reports2017
Integrating Extracellular Flux Measurements and Genome-Scale Modeling Reveals Differences between Brown and White Adipocytes.
Article in Cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- ceas: an R package for Seahorse data analysis and visualization.Bioinformatics (Oxford, England) · 2024Article
- Characterizing cancer metabolism from bulk and single-cell RNA-seq data using METAFlux.Nature communications · 2023Article
- Quantitative analysis of metabolic fluxes in brown fat and skeletal muscle during thermogenesis.Nature metabolism · 2023Article
- PAT2 regulates vATPase assembly and lysosomal acidification in brown adipocytes.Molecular metabolism · 2022Article
- Systems-based approaches to study immunometabolism.Cellular & molecular immunology · 2022Review
- The mitochondrial protein Opa1 promotes adipocyte browning that is dependent on urea cycle metabolites.Nature metabolism · 2021Article
- In vivo isotope tracing reveals the versatility of glucose as a brown adipose tissue substrate.Cell reports · 2021Article
- NetFlow: A tool for isolating carbon flows in genome-scale metabolic networks.Metabolic engineering communications · 2021Article
- Lipid and glucose metabolism in white adipocytes: pathways, dysfunction and therapeutics.Nature reviews. Endocrinology · 2021Review
- Computational deconvolution of synovial tissue cellular composition: presence of adipocytes in synovial tissue decreased during arthritis pathogenesis and progression.Physiological genomics · 2019Article
- Membrane metallo-endopeptidase (Neprilysin) regulates inflammatory response and insulin signaling in white preadipocytes.Molecular metabolism · 2019Article
- Substrate fluxes in brown adipocytes upon adrenergic stimulation and uncoupling protein 1 ablation.Life science alliance · 2018Article
- Squeezing Flux Out of Fat.Trends in endocrinology and metabolism: TEM · 2018Article
- An Untargeted Metabolomics Approach to Investigate the Metabolic Modulations of HepG2 Cells Exposed to Low Doses of Bisphenol A and 17β-Estradiol.Frontiers in endocrinology · 2018Article
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8 authors at 3 institutions in 1 country.
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Abstract
White adipocytes are specialized for energy storage, whereas brown adipocytes are specialized for energy expenditure. Explicating this difference can help identify therapeutic targets for obesity. A common tool to assess metabolic differences between such cells is the Seahorse Extracellular Flux (XF) Analyzer, which measures oxygen consumption and media acidification in the presence of different substrates and perturbagens. Here, we integrate the Analyzer's metabolic profile from human white and brown adipocytes with a genome-scale metabolic model to predict flux differences across the metabolic map. Predictions matched experimental data for the metabolite 4-aminobutyrate, the protein ABAT, and the fluxes for glucose, glutamine, and palmitate. We also uncovered a difference in how adipocytes dispose of nitrogenous waste, with brown adipocytes secreting less ammonia and more urea than white adipocytes. Thus, the method and software we developed allow for broader metabolic phenotyping and provide a distinct approach to uncovering metabolic differences.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.