ArticleASAIO journal (American Society for Artificial Internal Organs : 1992)
Orbital Shear Stress Regulates Differentiation and Barrier Function of Primary Renal Tubular Epithelial Cells.
Article in ASAIO journal (American Society for Artificial Internal Organs : 1992). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Design and Implementation of a Universal Donor Kidney.Kidney360 · 2026Review
- 3D Engineered Biomimetic Platform for Characterization of Collective Invasion, Tumor Emboli Formation, and Lymphatic Dissemination.IEEE open journal of engineering in medicine and biology · 2026Article
- Sustained alterations in proximal tubule gene expression in primary culture associate with HNF4A loss.Scientific reports · 2024Article
- Focus on oliguria during renal replacement therapy.Journal of anesthesia · 2024Review
- Feasibility of an implantable bioreactor for renal cell therapy using silicon nanopore membranes.Nature communications · 2023Article
- Making In Vitro Tumor Models Whole Again.Advanced healthcare materials · 2023Review
- Non-Invasive, Targeted Nanoparticle-Mediated Drug Delivery across a Novel Human BBB Model.Pharmaceutics · 2023Article
- Inhibition of Transforming Growth Factor-β Improves Primary Renal Tubule Cell Differentiation in Long-Term Culture.Tissue engineering. Part A · 2023Article
- Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalentsBiomaterials and biosystems · 2022Article
- Kidney-on-a-Chip: Mechanical Stimulation and Sensor Integration.Sensors (Basel, Switzerland) · 2022Review
- Functional Drug Screening using Kidney Cells On-A-Chip: Advances in Disease Modeling and Development of Biomarkers.Kidney360 · 2022Article
- Cell spinpods are a simple inexpensive suspension culture device to deliver fluid shear stress to renal proximal tubular cells.Scientific reports · 2021Article
- Bioengineered Kidney Models: Methods and Functional Assessments.Function (Oxford, England) · 2021Review
- Role of Shear Stress on Renal Proximal Tubular Cells for Nephrotoxicity Assays.Journal of toxicology · 2021Review
- "Microgravity" as a unique and useful stem cell culture environment for cell-based therapy.Regenerative therapy · 2019Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Primary cells cultured in vitro gradually lose features characteristic of the in vivo phenotype. Culture techniques that help maintain cell-specific phenotype are advantageous for development of tissue engineered and bioartificial organs. Here we evaluated the phenotype of primary human renal tubular epithelial cells subjected to fluid shear stress by culturing the cells on an orbital shaker. Transepithelial electrical resistance (TEER), cell density, and gene and protein expression of proximal tubule-specific functional markers were measured in cells subjected to orbital shear stress. Cells cultured on an orbital shaker had increased TEER, higher cell density, and enhanced tubular epithelial specific gene and protein expression. This is likely due at least in part to the mechanical stress applied to the apical surface of the cells although other factors including increased nutrient and oxygen delivery and improved mixing could also play a role. These results suggest that orbital shaker culture may be a simple approach to augmenting the differentiated phenotype of cultured renal epithelial cells.
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