Evidence map›Paper›PMID 29238899›Full record

ArticleMolecular and cellular biochemistry2018

Induction of apoptosis in human glioma cell lines of various grades through the ROS-mediated mitochondrial pathway and caspase activation by Rhaponticum carthamoides transformed root extract.

Ewa Skała, Tomasz Kowalczyk, Monika Toma, Janusz Szemraj, Maciej Radek, Dariusz Pytel, Joanna Wieczfinska, Halina Wysokińska, Tomasz Śliwiński, Przemysław Sitarek

Abstract read
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In one paragraph

Article in Molecular and cellular biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Diterpenoids fromPharmaceuticals (Basel, Switzerland) · 2020
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Oxidative medicine and cellular longevity · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ewa SkałaDepartment of Biology and Pharmaceutical Botany, Medical University of Łódź, Muszyńskiego 1, 90-151, Łódź, Poland. ewa.skala@umed.lodz.pl.
Tomasz KowalczykDepartment of Genetics and Plant Molecular Biology and Biotechnology, The University of Łódź, Banacha 12/13, 90-237, Łódź, Poland.
Monika TomaLaboratory of Medical Genetics, University of Łódź, Pomorska 141/143, 90-236, Łódź, Poland.
Janusz SzemrajDepartment of Medical Biochemistry, Medical University of Łódź, Mazowiecka 6/8, 92-215, Łódź, Poland.
Maciej RadekDepartment of Neurosurgery, Surgery of Spine and Peripheral Nerves, Medical University of Łódź, University Hospital WAM-CSW, Łódź, Poland.
Dariusz PytelDepartment of Biochemistry and Molecular Biology, Medical University of South Carolina, Hollings Cancer Center, HCC-709, 86 Jonathan Lucas Street, Charleston, SC, 29425, USA.
Joanna WieczfinskaDepartment of Immunopathology, Chair of Allergology, Immunology and Dermatology, Faculty of Biomedical Sciences and Postgraduate Training, Medical University of Łódź, Żeligowskiego 7/9, 90-752, Łódź, Poland.
Halina WysokińskaDepartment of Biology and Pharmaceutical Botany, Medical University of Łódź, Muszyńskiego 1, 90-151, Łódź, Poland.
Tomasz ŚliwińskiLaboratory of Medical Genetics, University of Łódź, Pomorska 141/143, 90-236, Łódź, Poland.
Przemysław SitarekDepartment of Biology and Pharmaceutical Botany, Medical University of Łódź, Muszyńskiego 1, 90-151, Łódź, Poland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study is the first investigation of the inhibitory effect of Rhaponticum carthamoides transformed roots (TR) extract on the proliferation of grade II and III human glioma cells. TR extract showed the cytotoxic effect and inhibited the colony formation of both glioma cell lines in dose-dependent manner. The root extract induced apoptosis by increasing of the reactive oxygen species (about threefold compared to the control cells) leading to a disruption of mitochondrial membrane potential. Additionally, the mRNA levels of the apoptotic factors such as Bax, Tp53, caspase-3, and caspase-9 were observed to increase. These results indicate that the TR extract possesses anticancer activity by inhibiting glioma cell proliferation and inducing apoptotic cell death, and may be used as a promising anticancer agent.

Indexed as

Antineoplastic Agents, Phytogenicbcl-2-Associated X ProteinBrain NeoplasmsCaspase 3Caspase 9Cell Line, TumorCell ProliferationEnzyme ActivationGliomaHumansLeuzeaMembrane Potential, MitochondrialMiddle AgedPlant ExtractsPlant RootsReactive Oxygen SpeciesAntineoplastic Agents, Phytogenicbcl-2-Associated X ProteinCaspase 3Caspase 9Plant ExtractsReactive Oxygen SpeciesTP53 protein, humanTumor Suppressor Protein p53ApoptosisBaxBcl-2Caffeic acid derivativesCaspase-3Caspase-9 gene expressionsHuman glioma cellsRhaponticum carthamoidesTp53Transformed roots

Identifiers

PMID29238899

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.