ArticleIranian journal of microbiology2017
Cloning, expression and purification of a polytopic antigen comprising of surface antigens of
Article in Iranian journal of microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Label-free quantitative proteomic analysis of ethanamizuril-resistant versus -sensitive strains of Eimeria tenella.Parasites & vectors · 2022Article
- Different strategies for expression and purification of the CT26-poly-neoepitopes vaccine in Escherichia coli.Molecular biology reports · 2022Article
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3 authors.
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Abstract
BACKGROUND AND
objectivesPolytopic antigens are recently applied for replacing crude antigens, for control of infectious agents. The surface of the Toxoplasma is covered with immunogenic antigens namely surface antigens (SAGs). These antigens possess several immunogenic epitopes, inducing immune responses. MATERIALS AND
methodsIn this study, a DNA construct comprising of sequences encoding epitopes from SAG1, 2 and 3 was designed and cloned into pET28a expression vector and subsequently expressed and purified, using Ni-NTA column.
resultsThe SDS-PAGE and Western blotting analysis showed that polytopic genes were successfully expressed and purified.
conclusionThe surface antigenic protein of
Indexed as
Identifiers
29238461PMC5723978What OpenQuestion holds
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