ArticlePloS one2017
Chondrocytes and stem cells in 3D-bioprinted structures create human cartilage in vivo.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
60 citing papers in PubMed, 3 syntheses or guidelines pooled it, 120 citations in OpenAlex.
- The Application of Cartilage Tissue Engineering with Cell-Laden Hydrogel in Plastic Surgery: A Systematic Review.Tissue engineering and regenerative medicine · 2022Pooled it
- Three-Dimensional Bioprinting Scaffolding for Nasal Cartilage Defects: A Systematic Review.Tissue engineering and regenerative medicine · 2021Pooled it
- Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior.Frontiers in bioengineering and biotechnology · 2020Pooled it
- GelMA Hydrogel/Alginate-Based Scaffolds: 3D Bioprinting for Cartilage Tissue Engineering.Tissue engineering and regenerative medicine · 2026Article
- 3D-bioprinting for joint regeneration.Frontiers in bioengineering and biotechnology · 2026Review
- Cell Co-Implantations With Chondrocytes for Cartilage Repair: A Systematic Review ofCartilage · 2025Article
- Differentiation of stem cells into chondrocytes and their potential clinical application in cartilage regeneration.Histochemistry and cell biology · 2025Review
- Article
- 3D-printed constructs deliver bioactive cargos to expedite cartilage regeneration.Journal of pharmaceutical analysis · 2024Review
- Human Septal Cartilage Tissue Engineering: Current Methodologies and Future Directions.Bioengineering (Basel, Switzerland) · 2024Review
- 3D Bioprinting of Hyaline Cartilage Using Nasal Chondrocytes.Annals of biomedical engineering · 2024Review
- A Contemporary Review of Trachea, Nose, and Ear Cartilage Bioengineering and Additive Manufacturing.Biomimetics (Basel, Switzerland) · 2024Review
- Integrating bioprinting, cell therapies and drug delivery towards in vivo regeneration of cartilage, bone and osteochondral tissue.Drug delivery and translational research · 2024Review
- Orthotopic equine study confirms the pivotal importance of structural reinforcement over the pre-culture of cartilage implants.Bioengineering & translational medicine · 2024Article
- 4-Axis 3D-Printed Tubular Biomaterials Imitating the Anisotropic Nanofiber Orientation of Porcine Aortae.Advanced healthcare materials · 2024Article
- Adipose-Derived Stromal Cells Preserve Pancreatic Islet Function in a Transplantable 3D Bioprinted Scaffold.Advanced healthcare materials · 2023Article
- 3D Bioprinting in Otolaryngology: A Review.Advanced healthcare materials · 2023Review
- Cartilage 3D bioprinting for rhinoplasty using adipose-derived stem cells as seed cells: Review and recent advances.Cell proliferation · 2023Review
- A Psychrophilic GelMA: Breaking Technical and Immunological Barriers for Multimaterial High-Resolution 3D Bioprinting.Biomacromolecules · 2023Article
- Development and characterization of an automated active mixing platform for hydrogel bioink preparation.International journal of bioprinting · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cartilage repair and replacement is a major challenge in plastic reconstructive surgery. The development of a process capable of creating a patient-specific cartilage framework would be a major breakthrough. Here, we described methods for creating human cartilage in vivo and quantitatively assessing the proliferative capacity and cartilage-formation ability in mono- and co-cultures of human chondrocytes and human mesenchymal stem cells in a three-dimensional (3D)-bioprinted hydrogel scaffold. The 3D-bioprinted constructs (5 × 5 × 1.2 mm) were produced using nanofibrillated cellulose and alginate in combination with human chondrocytes and human mesenchymal stem cells using a 3D-extrusion bioprinter. Immediately following bioprinting, the constructs were implanted subcutaneously on the back of 48 nude mice and explanted after 30 and 60 days, respectively, for morphological and immunohistochemical examination. During explantation, the constructs were easy to handle, and the majority had retained their macroscopic grid appearance. Constructs consisting of human nasal chondrocytes showed good proliferation ability, with 17.2% of the surface areas covered with proliferating chondrocytes after 60 days. In constructs comprising a mixture of chondrocytes and stem cells, an additional proliferative effect was observed involving chondrocyte production of glycosaminoglycans and type 2 collagen. This clinically highly relevant study revealed 3D bioprinting as a promising technology for the creation of human cartilage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.