ArticlePloS one2017
Stability evaluation of reference genes for gene expression analysis by RT-qPCR in soybean under different conditions.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed.
- A novel DREB transcription factor enhances salt tolerance in peanut (Arachis hypogaea L.) through upregulation of AhP5CR and AhBADH, leading to increased osmolyte accumulation.Molecular biology reports · 2026Article
- Identification and validation of reference genes for quantitative real-time PCR in Corydalis saxicola under various abiotic stresses.Scientific reports · 2026Article
- Selection and validation of suitable reference genes for qPCR expression analysis in youngFrontiers in plant science · 2026Article
- Lipidomics reveals divergent root and shoot responses to combined nitrogen deficiency and heat stress.Frontiers in plant science · 2026Article
- Molecular Mechanisms and Crosstalk Signaling in Soybean's Response to Water Deficit and Excess: Implications for Stress Resilience and Productivity.Plants (Basel, Switzerland) · 2025Article
- Article
- Assessment of potential candidate genes for partial resistance to Sclerotinia stem rot caused by Sclerotinia sclerotiorum using real-time quantitative PCR.The plant genome · 2025Article
- Yield and yield component trait analysis with DArT genotyping for GWAS in soybean grown in drought conditions of Kazakhstan.Frontiers in plant science · 2025Article
- Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review.International journal of molecular sciences · 2024Review
- Article
- Identification of Reference Genes for Precise Expression Analysis during Germination inInternational journal of molecular sciences · 2023Article
- Noninvasive Methods to Detect Reactive Oxygen Species as a Proxy of Seed Quality.Antioxidants (Basel, Switzerland) · 2023Article
- I Choose You: Selecting Accurate Reference Genes for qPCR Expression Analysis in Reproductive Tissues inBiomolecules · 2023Article
- Article
- Constitutive expression of a pea apyrase, psNTP9, increases seed yield in field-grown soybean.Scientific reports · 2022Article
- Reference Genes and Expression Analysis of Seed Desaturases Genes in Soybean Mutant Accessions.Biochemical genetics · 2022Article
- Effects of a multicomponent microbial feed additive containing prebiotics and probiotics on health, immune status, metabolism, and performance of newly weaned beef steers during a 35-d receiving period.Translational animal science · 2022Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Normalization inFrontiers in physiology · 2022Article
- Recent advancement in OMICS approaches to enhance abiotic stress tolerance in legumes.Frontiers in plant science · 2022Review
- Validation of reference genes for quantitative real-time PCR in chemical exposed and at different age's brackish water flea Diaphanosoma celebensis.Scientific reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time quantitative reverse transcription PCR is a sensitive and widely used technique to quantify gene expression. To achieve a reliable result, appropriate reference genes are highly required for normalization of transcripts in different samples. In this study, 9 previously published reference genes (60S, Fbox, ELF1A, ELF1B, ACT11, TUA5, UBC4, G6PD, CYP2) of soybean [Glycine max (L.) Merr.] were selected. The expression stability of the 9 genes was evaluated under conditions of biotic stress caused by infection with soybean mosaic virus, nitrogen stress, across different cultivars and developmental stages. ΔCt and geNorm algorithms were used to evaluate and rank the expression stability of the 9 reference genes. Results obtained from two algorithms showed high consistency. Moreover, results of pairwise variation showed that two reference genes were sufficient to normalize the expression levels of target genes under each experimental setting. For virus infection, ELF1A and ELF1B were the most stable reference genes for accurate normalization. For different developmental stages, Fbox and G6PD had the highest expression stability between two soybean cultivars (Tanlong No. 1 and Tanlong No. 2). ELF1B and ACT11 were identified as the most stably expressed reference genes both under nitrogen stress and among different cultivars. The results showed that none of the candidate reference genes were uniformly expressed at different conditions, and selecting appropriate reference genes was pivotal for gene expression studies with particular condition and tissue. The most stable combination of genes identified in this study will help to achieve more accurate and reliable results in a wide variety of samples in soybean.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.