ArticleBioTechniques2017
Processing fixed and stored adipose-derived stem cells for quantitative protein array assays.
Article in BioTechniques, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Generating Cell Type-Specific Protein Signatures from Non-symptomatic and Diseased Tissues.Annals of biomedical engineering · 2020Pooled it
- Discovery of surface biomarkers for cell mechanophenotype via an intracellular protein-based enrichment strategy.Cellular and molecular life sciences : CMLS · 2022Article
- Mass-Added Density Modulation for Sorting Cells Based on Differential Surface Protein Levels.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Accurately characterizing cellular subpopulations is essential for elucidating the mechanisms underlying normal and pathological biology. Isolation of specific cell types can be accomplished by labeling unique cell-associated proteins with fluorescent antibodies. Cell fixation is commonly used to prepare these samples and allow for long-term storage, but this poses challenges for subsequent protein analysis. We previously established the FITSAR (formaldehyde-fixed intracellular target-sorted antigen retrieval) method, in which protein can be isolated and characterized from fixed, enriched cell subpopulations. Here, we improve on this method by allowing compatibility with highly sensitive multiplex protein arrays and demonstrating applicability to long-term stored samples. Feasibility experiments demonstrated parallel detection of cell adhesion molecules (CAMs) using an enzyme-linked immunosorbent assay (ELISA) panel with human adipose-derived stem cells (ASCs) stored for up to 1 month.
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Registered trials
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