ArticleScientific reports2017
Metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium Synechocysti s sp. PCC 6803.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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55 citing papers in PubMed, 122 citations in OpenAlex.
- Enhancing Geranylgeranyl Pyrophosphate Supply in Engineered Cyanobacteria for Improved Production of Natural Pigments and Terpenoid Precursors.International journal of molecular sciences · 2026Article
- The Mevalonate Pathway: Innovations, Applications, and Challenges in Biotechnology with Emphasis on Fungal Biology.Journal of fungi (Basel, Switzerland) · 2026Review
- From pond to platform: howJournal of bacteriology · 2026Review
- Fermi calculations enable quick downselection of target genes and process optimization in photosynthetic systems.Plant physiology · 2025Review
- The Sustainable Production of Terpenoids in Cyanobacterial Chassis.Microorganisms · 2025Review
- Effect of Culture Temperature on 2-Methylisoborneol Production and Gene Expression in Two Strains ofCells · 2024Article
- Nutraceutical prospects of genetically engineered cyanobacteria- technological updates and significance.World journal of microbiology & biotechnology · 2024Review
- Application of Cyanobacteria as Chassis Cells in Synthetic Biology.Microorganisms · 2024Review
- Impact of Carbon Fixation, Distribution and Storage on the Production of Farnesene and Limonene inInternational journal of molecular sciences · 2024Article
- Two-Phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes.Molecules (Basel, Switzerland) · 2024Review
- Increasing the Pentose Phosphate Pathway Flux to Improve Plasmid DNA Production in EngineeredMicroorganisms · 2024Article
- Development ofFrontiers in microbiology · 2024Article
- Application of valencene and prospects for its production in engineered microorganisms.Frontiers in microbiology · 2024Review
- Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria.Microorganisms · 2023Review
- A systematic overexpression approach reveals native targets to increase squalene production inFrontiers in plant science · 2023Article
- Metabolic Engineering Design Strategies for Increasing Carbon Fluxes Relevant for Biosynthesis in Cyanobacteria.Advances in biochemical engineering/biotechnology · 2023Article
- Exploring the potential of the model cyanobacterium Synechocystis PCC 6803 for the photosynthetic production of various high-value terpenes.Biotechnology for biofuels and bioproducts · 2022Article
- Metabolic Engineering of the Isopentenol Utilization Pathway Enhanced the Production of Terpenoids inMarine drugs · 2022Article
- Engineering plant family TPS into cyanobacterial host for terpenoids production.Plant cell reports · 2022Review
- Thermodynamics contributes to high limonene productivity in cyanobacteria.Metabolic engineering communications · 2022Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isoprenoids are diverse natural compounds, which have various applications as pharmaceuticals, fragrances, and solvents. The low yield of isoprenoids in plants makes them difficult for cost-effective production, and chemical synthesis of complex isoprenoids is impractical. Microbial production of isoprenoids has been considered as a promising approach to increase the yield. In this study, we engineered the model cyanobacterium Synechocystis sp. PCC 6803 for sustainable production of a commercially valuable isoprenoid, limonene. Limonene synthases from the plants Mentha spicata and Citrus limon were expressed in cyanobacteria for limonene production. Production of limonene was two-fold higher with limonene synthase from M. spicata than that from C. limon. To enhance isoprenoid production, computational strain design was conducted by applying the OptForce strain design algorithm on Synechocystis 6803. Based on the metabolic interventions suggested by this algorithm, genes (ribose 5-phosphate isomerase and ribulose 5-phosphate 3-epimerase) in the pentose phosphate pathway were overexpressed, and a geranyl diphosphate synthase from the plant Abies grandis was expressed to optimize the limonene biosynthetic pathway. The optimized strain produced 6.7 mg/L of limonene, a 2.3-fold improvement in productivity. Thus, this study presents a feasible strategy to engineer cyanobacteria for photosynthetic production of isoprenoids.
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