ArticleScientific reports2017
Inactivation of the dnaK gene in Clostridium difficile 630 Δerm yields a temperature-sensitive phenotype and increases biofilm-forming ability.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 46 citations in OpenAlex.
- Disruption ofJournal of medical microbiology · 2026Article
- Protein Homeostasis Impairment Alters Phenotypic Heterogeneity of Biofilm Communities.Molecular microbiology · 2025Article
- Extracellular DNA filaments associated with surface polysaccharide II give Clostridioides difficile biofilm matrix a network-like structure.NPJ biofilms and microbiomes · 2025Article
- The DnaJK chaperone ofmBio · 2025Article
- Age-Dependent Pleomorphism inMicroorganisms · 2025Article
- Multiple Chaperone DnaK-FliC Flagellin Interactions are Required for Pseudomonas aeruginosa Flagellum Assembly and Indicate a New Function for DnaK.Microbial biotechnology · 2025Article
- The Importance of Maintaining and Improving a Healthy Gut Microbiota in Athletes as a Preventive Strategy to Improve Heat Tolerance and Acclimatization.Microorganisms · 2024Review
- Sortase-encoding genes,Frontiers in microbiology · 2024Article
- Pathogenicity and virulence ofVirulence · 2023Review
- Review of the Impact of Biofilm Formation on RecurrentMicroorganisms · 2023Review
- Characterization of Molecular Chaperone GroEL as a Potential Virulence Factor inFoods (Basel, Switzerland) · 2023Article
- Biofilm Formation ofMicroorganisms · 2023Review
- Non-Toxin-BasedPathogens (Basel, Switzerland) · 2023Article
- Insight into the mechanism of gallstone disease by proteomic and metaproteomic characterization of human bile.Frontiers in microbiology · 2023Article
- Efficacy of Dry Heat Treatment againstPathogens (Basel, Switzerland) · 2022Article
- Utilizing a reductionist model to study host-microbe interactions in intestinal inflammation.Microbiome · 2021Article
- Blowing Hot and Cold: Body Temperature and the Microbiome.mSystems · 2021Article
- Wolf in Sheep's Clothing:Microorganisms · 2021Review
- Biofilm regulation in Clostridioides difficile: Novel systems linked to hypervirulence.PLoS pathogens · 2021Review
- Mucin-Degrading Microbes Release Monosaccharides That ChemoattractACS infectious diseases · 2021Article
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7 authors at 4 institutions in 1 country.
Funding
Abstract
Clostridium difficile infection is a growing problem in healthcare settings worldwide and results in a considerable socioeconomic impact. New hypervirulent strains and acquisition of antibiotic resistance exacerbates pathogenesis; however, the survival strategy of C. difficile in the challenging gut environment still remains incompletely understood. We previously reported that clinically relevant heat-stress (37-41 °C) resulted in a classical heat-stress response with up-regulation of cellular chaperones. We used ClosTron to construct an insertional mutation in the dnaK gene of C. difficile 630 Δerm. The dnaK mutant exhibited temperature sensitivity, grew more slowly than C. difficile 630 Δerm and was less thermotolerant. Furthermore, the mutant was non-motile, had 4-fold lower expression of the fliC gene and lacked flagella on the cell surface. Mutant cells were some 50% longer than parental strain cells, and at optimal growth temperatures, they exhibited a 4-fold increase in the expression of class I chaperone genes including GroEL and GroES. Increased chaperone expression, in addition to the non-flagellated phenotype of the mutant, may account for the increased biofilm formation observed. Overall, the phenotype resulting from dnaK disruption is more akin to that observed in Escherichia coli dnaK mutants, rather than those in the Gram-positive model organism Bacillus subtilis.
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