ReviewFrontiers in molecular biosciences2017
Technical Advances to Study Extracellular Vesicles.
Review in Frontiers in molecular biosciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Efficient Labeling of Vesicles with Lipophilic Fluorescent Dyes via the Salt-Change Method.Analytical chemistry · 2023Article
- Real-Time Dissection of the Transportation and miRNA-Release Dynamics of Small Extracellular Vesicles.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Two Complementary Strategies to Quantitate Extracellular Vesicle Uptake Using Bioluminescence and Non-Lipidic Dyes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Editorial: The role of extracellular vesicles in diseases: Shedding light on their role in cell-to-cell communication.Frontiers in genetics · 2023Article
- Extracellular vesicles and Alzheimer's disease in the novel era of Precision Medicine: implications for disease progression, diagnosis and treatment.Experimental neurology · 2022Review
- Selective Isolation of Liver-Derived Extracellular Vesicles Redefines Performance of miRNA Biomarkers for Non-Alcoholic Fatty Liver Disease.Biomedicines · 2022Article
- Coupling size exclusion chromatography to ultracentrifugation improves detection of exosomal proteins from human plasma by LC-MS.Practical laboratory medicine · 2021Article
- Autophagy and Glycative Stress: A Bittersweet Relationship in Neurodegeneration.Frontiers in cell and developmental biology · 2021Review
- Extracellular Vesicles: Potential Role in Remote Signaling and Inflammation inFrontiers in cell and developmental biology · 2021Review
- Novel insights for improving the therapeutic safety and efficiency of mesenchymal stromal cells.World journal of stem cells · 2020Review
- Tumor-derived exosomes: the next generation of promising cell-free vaccines in cancer immunotherapy.Oncoimmunology · 2020Review
- Article
- Spatial and temporal tracking of cardiac exosomes in mouse using a nano-luciferase-CD63 fusion protein.Communications biology · 2020Article
- Harnessing the Neural Stem Cell Secretome for Regenerative Neuroimmunology.Frontiers in cellular neuroscience · 2020Review
- Development of a quantitative method to measure EV uptake.Scientific reports · 2019Article
- TLR4 participates in the transmission of ethanol-induced neuroinflammation via astrocyte-derived extracellular vesicles.Journal of neuroinflammation · 2019Article
- Extracellular RNA in viral-host interactions: Thinking outside the cell.Wiley interdisciplinary reviews. RNA · 2019Review
- Delivery of microRNAs by Extracellular Vesicles in Viral Infections: Could the News be Packaged?Cells · 2019Review
- FASN activity is important for the initial stages of the induction of senescence.Cell death & disease · 2019Article
- Extracellular Vesicles in Psychiatry Research in the Context of RDoC Criteria.Psychiatry investigation · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular vesicles are a heterogeneous and dynamic group of lipid bilayer membrane nanoparticles that can be classified into three different groups depending on their cellular origin: exosomes, microvesicles, and apoptotic bodies. They are produced by different cell types and can be isolated from almost all body fluids. EVs contain a variety of proteins, lipids, nucleic acids, and metabolites which regulate a number of biological and pathological scenarios both locally and systemically. Different techniques have been described in order to determine EV isolation, release, uptake, and cargo. Although standard techniques such as immunoblotting, fluorescent microscopy, and electron microscopy are still being used to characterize and visualize EVs, in the last years, more fine-tuned techniques are emerging. For example, EV uptake can be specifically determined at a single cell level using the Cre reporter methodology and bioluminescence based-methods reports have been employed to determine both EV release and uptake. In addition, techniques for cargo identification have also enormously evolved during these years. Classical mass spectrometry and next generation sequencing have been used in the past, but nowadays, advances in these tools have facilitated a more in depth characterization of the EV content. In this review, we aim to assess the standard and latest technical advances for studying EV biology in different biological systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.