ArticlePlant methods2017
Robust genetic transformation of sorghum (
Article in Plant methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 59 citations in OpenAlex.
- Biotechnological and genetic innovations to enhance sorghum adaptation under climate change.Frontiers in plant science · 2026Review
- Optimized Protocol for Sorghum Regeneration: Enhancing Embryogenic Callus Formation from Immature Inflorescences.Recent patents on biotechnology · 2026Article
- Article
- Combined Analysis of BSA-Seq and RNA-Seq Reveals Candidate Genes forPlants (Basel, Switzerland) · 2025Article
- Adapting CProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Association mapping and identification of candidate genes for callus induction and regeneration using sorghum mature seeds.Frontiers in plant science · 2025Article
- Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery.The Plant journal : for cell and molecular biology · 2025Article
- Construction and Validation of CRISPR/Cas Vectors for Editing theCurrent issues in molecular biology · 2024Article
- Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing.International journal of molecular sciences · 2023Review
- Technological Development and Application of Plant Genetic Transformation.International journal of molecular sciences · 2023Review
- New methods for sorghum transformation in temperate climates.AoB PLANTS · 2023Review
- Uncovering transcriptional reprogramming during callus development in soybean: insights and implications.Frontiers in plant science · 2023Article
- Recent advancements in the breeding of sorghum crop: current status and future strategies for marker-assisted breeding.Frontiers in genetics · 2023Review
- Article
- Beyond a reference genome: pangenomes and population genomics of underutilized and orphan crops for future food and nutrition security.The New phytologist · 2022Review
- Progress and challenges in sorghum biotechnology, a multipurpose feedstock for the bioeconomy.Journal of experimental botany · 2022Review
- An efficient sorghum transformation system using embryogenic calli derived from mature seeds.PeerJ · 2021Article
- Particle bombardment technology and its applications in plants.Molecular biology reports · 2020Review
- Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes.Plants (Basel, Switzerland) · 2020Article
- Stable and Efficient Agrobacterium-Mediated Genetic Transformation of Larch Using Embryogenic Callus.Frontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSorghum (
resultsWe report here a robust genetic transformation method for sorghum (Tx430) using differentiating embryogenic calli (DEC) with nodular structures induced from immature embryos and maintained for more than a year without losing regeneration potential on modified MS media. The addition of lipoic acid (LA) to callus induction media along with optimized growth regulators increased callus induction frequency from 61.3 ± 3.2 to 79 ± 6.5% from immature embryos (1.5-2.0 mm in length) isolated 12-15 days after pollination. Similarly, the regeneration efficiency and the number of shoots from DEC tissue was enhanced by LA. The optimized regeneration system in combination with particle bombardment resulted in an average transformation efficiency (TE) of 27.2 or 46.6% based on the selection strategy, 25% to twofold higher TE than published reports in Tx430. Up to 100% putative transgenic shoots were positive for
conclusionsThis protocol demonstrates significant improvements in both efficiency and ease of use over existing sorghum transformation methods using PDS, also enables quick hypothesis testing in the production of various high value products in sorghum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.