ArticleStem cell reports2018
Generation of Adrenal Chromaffin-like Cells from Human Pluripotent Stem Cells.
Article in Stem cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Differentiation of human embryonic stem cells into adrenal chromaffin-like cells with catecholamine markers, excitability, and CaSTAR protocols · 2026Article
- Long-term culture of patient-derived pheochromocytoma organoids.Frontiers in endocrinology · 2026Article
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- Canine Adrenomedullary and Pheochromocytoma Organoids: A Novel In Vitro Model.Endocrinology · 2025Article
- Comparison of human pluripotent stem cell differentiation protocols to generate neuroblastoma tumors.Scientific reports · 2024Article
- The adipose-neural axis is involved in epicardial adipose tissue-related cardiac arrhythmias.Cell reports. Medicine · 2024Article
- Human iPSC modeling recapitulatesiScience · 2024Article
- Forty years of the adrenal chromaffin cell through ISCCB meetings around the world.Pflugers Archiv : European journal of physiology · 2023Review
- Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor.eLife · 2022Article
- Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells.Biochemical Society transactions · 2022Article
- From head to tail: regionalization of the neural crest.Development (Cambridge, England) · 2020Review
- Retinoic Acid Accelerates the Specification of Enteric Neural Progenitors from In-Vitro-Derived Neural Crest.Stem cell reports · 2020Article
- Efficient Generation of Trunk Neural Crest and Sympathetic Neurons from Human Pluripotent Stem Cells Via a Neuromesodermal Axial Progenitor Intermediate.Current protocols in stem cell biology · 2019Article
- FGF Modulates the Axial Identity of Trunk hPSC-Derived Neural Crest but Not the Cranial-Trunk Decision.Stem cell reports · 2019Article
- Stem Cells, Self-Renewal, and Lineage Commitment in the Endocrine System.Frontiers in endocrinology · 2019Review
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adrenomedullary chromaffin cells are catecholamine (CA)-producing cells originating from trunk neural crest (NC) via sympathoadrenal progenitors (SAPs). We generated NC and SAPs from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in vitro via BMP2/FGF2 exposure, ascertained by qPCR and immunoexpression of SOX10, ASCL1, TFAP2α, and PHOX2B, and by fluorescence-activated cell sorting selection for p75NTR and GD2, and confirmed their trunk-like HOX gene expression. We showed that continuing BMP4 and curtailing FGF2 in vitro, augmented with corticosteroid mimetic, induced these cells to upregulate the chromaffin cell-specific marker PNMT and other CA synthesis and storage markers, and we demonstrated noradrenaline and adrenaline by Faglu and high-performance liquid chromatography. We showed these human cells' SAP-like property of migration and differentiation into cells expressing chromaffin cell markers by implanting them into avian embryos in vivo and in chorio-allantoic membrane grafts. These cells have the potential for investigating differentiation of human chromaffin cells and for modeling diseases involving this cell type.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.