ArticleBMC plant biology2017
De novo transcriptome assembly from flower buds of dioecious, gynomonoecious and chemically masculinized female Coccinia grandis reveals genes associated with sex expression and modification.
Article in BMC plant biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
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- Anatomical and Ontogenetic Basis of Silver Thiosulfate-Induced Masculinization in Genetically FemalePlants (Basel, Switzerland) · 2026Article
- The Mechanism of the Development and Maintenance of Sexual Dimorphism in the Dioecious Mulberry Plant (Biology · 2024Article
- A Novel Method for StimulatingPlants (Basel, Switzerland) · 2023Article
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- The evolution of huge Y chromosomes inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Comparative RNA-Seq analysis reveals genes associated with masculinization in female Cannabis sativa.Planta · 2021Article
- Production of Feminized Seeds of High CBDFrontiers in plant science · 2021Article
- Development of a Virus-Induced Gene Silencing System for Dioecious Coccinia grandis.Molecular biotechnology · 2020Article
- Evidence for Dosage Compensation inGenes · 2020Article
- Transcriptome profiling of differentially expressed genes in cytoplasmic male-sterile line and its fertility restorer line in pigeon pea (Cajanus cajan L.).BMC plant biology · 2020Article
- Flower bud proteome reveals modulation of sex-biased proteins potentially associated with sex expression and modification in dioecious Coccinia grandis.BMC plant biology · 2019Article
- Sex and the flower - developmental aspects of sex chromosome evolution.Annals of botany · 2018Review
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundCoccinia grandis (ivy gourd), is a dioecious member of Cucurbitaceae having heteromorphic sex chromosomes. Chromosome constitution of male and female plants of C. grandis is 22A + XY and 22A + XX respectively. Earlier we showed that a unique gynomonoecious form of C. grandis (22A + XX) also exists in nature bearing morphologically hermaphrodite flowers (GyM-H). Additionally, application of silver nitrate (AgNO
resultsWe have carried out a comprehensive RNA-Seq study from early-staged male, female, GyM-H, and Ag-H as well as middle-staged male and GyM-H flower buds. A de novo transcriptome was assembled using Trinity and annotated by BLAST2GO and Trinotate pipelines. The assembled transcriptome consisted of 467,233 'Trinity Transcripts' clustering into 378,860 'Trinity Genes'. Female_Early_vs_Male_Early, Ag_Early_vs_Female_Early, and GyM-H_Middle_vs_Male_Middle comparisons exhibited 35,694, 3574, and 14,954 differentially expressed transcripts respectively. Further, qRT-PCR analysis of selected candidate genes validated digital gene expression profiling results. Interestingly, ethylene response-related genes were found to be upregulated in female buds compared to male buds. Also, we observed that AgNO
conclusionsSuppression of ethylene responses in both male and Ag-H compared to female buds suggests a probable role of ethylene in stamen suppression similar to monoecious cucurbits such as melon and cucumber. Also, pollen fertility associated GO terms were depleted in middle-staged GyM-H buds compared to male buds indicating the necessity of Y-chromosome for pollen fertility. Overall, this study would enable identification of new sex-biased genes for further investigation of stamen arrest, pollen fertility, and AgNO
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