Evidence map›Paper›PMID 29232996›Full record

ArticleEuropean journal of mass spectrometry (Chichester, England)2018

Structural elucidation of fucosylated chondroitin sulfates from sea cucumber using FTICR-MS/MS.

Isaac Agyekum, Lauren Pepi, Yanlei Yu, Junhui Li, Lufeng Yan, Robert J Linhardt, Shiguo Chen, I Jonathan Amster

Open access · greenAbstract readEvaluation Study
In one paragraph

Article in European journal of mass spectrometry (Chichester, England), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. Isolation and Compositional Analysis of Glycosaminoglycans.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Isaac Agyekum1 Department of Chemistry, University of Georgia, Athens, USA.
Lauren Pepi1 Department of Chemistry, University of Georgia, Athens, USA.
Yanlei Yu2 Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, 8024 Rensselaer Polytechnic Institute , Troy, USA.
Junhui Li3 Department of Food Science and Nutrition, Zhejiang University, China.
Lufeng Yan3 Department of Food Science and Nutrition, Zhejiang University, China.
Robert J Linhardt2 Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, 8024 Rensselaer Polytechnic Institute , Troy, USA.
Shiguo Chen3 Department of Food Science and Nutrition, Zhejiang University, China.
I Jonathan Amster1 Department of Chemistry, University of Georgia, Athens, USA.ORCID http://orcid.org/0000-0001-7523-5144
University of Georgia · USZhejiang University · CNRensselaer Polytechnic Institute · US

Funding

TR&D3. Approaches to Proteoglycan Function at Biochemical and Cellular LevelsP41GM103390 · NIGMS · UNIVERSITY OF GEORGIA · PI MOREMEN, KELLEY W. · 2012 to 2019
$14.5M
NIGMS NIH HHS P41 GM103390
6 · The paper itself

Abstract

Fucosylated chondroitin sulfates are complex polysaccharides extracted from sea cucumber. They have been extensively studied for their anticoagulant properties and have been implicated in other biological activities. While nuclear magnetic resonance spectroscopy has been used to extensively characterize fucosylated chondroitin sulfate oligomers, we herein report the first detailed mass characterization of fucosylated chondroitin sulfate using high-resolution Fourier transform ion cyclotron resonance mass spectrometry. The two species of fucosylated chondroitin sulfates considered for this work include Pearsonothuria graeffei (FCS-Pg) and Isostichopus badionotus (FCS-Ib). Fucosylated chondroitin sulfate oligosaccharides were prepared by N-deacetylation-deaminative cleavage of the two fucosylated chondroitin sulfates and purified by repeated gel filtration. Accurate mass measurements obtained from electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry measurements confirmed the oligomeric nature of these two fucosylated chondroitin sulfate oligosaccharides with each trisaccharide repeating unit averaging four sulfates per trisaccharide. Collision-induced dissociation of efficiently deprotonated molecular ions through Na/H

Indexed as

AnimalsAnticoagulantsChondroitin SulfatesFourier AnalysisKineticsMolecular StructureSea CucumbersTandem Mass SpectrometryAnticoagulantsChondroitin Sulfatesfucosylated chondroitin sulfatecarbohydratesFTICR-MS/MSfucosylated chondroitin sulfatesea cucumber

Identifiers

PMID29232996
PMCPMC5732082
OpenAlexW2775402656

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.