Evidence map›Paper›PMID 29232483›Full record

ArticleChembiochem : a European journal of chemical biology2018

Epitope-Resolved Detection of Peanut-Specific IgE Antibodies by Surface Plasmon Resonance Imaging.

Min Shen, Amit A Joshi, Raghu Vannam, Chandra K Dixit, Robert G Hamilton, Challa V Kumar, James F Rusling, Mark W Peczuh

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Min ShenDepartment of Chemistry, University of Connecticut, Storrs, CT, 06269, USA.
Amit A JoshiDepartment of Chemistry, University of Connecticut, Storrs, CT, 06269, USA.
Raghu VannamDepartment of Chemistry, University of Connecticut, Storrs, CT, 06269, USA.
Chandra K DixitDepartment of Chemistry, University of Connecticut, Storrs, CT, 06269, USA.
Robert G HamiltonDivision of Allergy and Clinical Immunology, Johns Hopkins University School of Medicine, Baltimore, MD, 21224, USA.
Challa V KumarDepartment of Chemistry, University of Connecticut, Storrs, CT, 06269, USA.ORCID 0000-0002-8399-1334
James F RuslingDepartment of Chemistry, University of Connecticut, Storrs, CT, 06269, USA.
Mark W PeczuhDepartment of Chemistry, University of Connecticut, Storrs, CT, 06269, USA.ORCID 0000-0002-3028-6551
University of Connecticut · USJohns Hopkins University · US

Funding

Protein Biomarker Arrays for Personalized Treatment of Prostate CancerR01EB016707 · NIBIB · UNIVERSITY OF CONNECTICUT STORRS · PI RUSLING, JAMES F. · 2014 to 2017
$1.4M
NIBIB NIH HHS R01 EB016707
6 · The paper itself

Abstract

Peanut allergy can be life-threatening and is mediated by allergen-specific immunoglobulin E (IgE) antibodies. Investigation of IgE antibody binding to allergenic epitopes can identify specific interactions underlying the allergic response. Here, we report a surface plasmon resonance imaging (SPRi) immunoassay for differentiating IgE antibodies by epitope-resolved detection. IgE antibodies were first captured by magnetic beads bearing IgE ϵ-chain-specific antibodies and then introduced into an SPRi array immobilized with epitopes from the major peanut allergen glycoprotein Arachis hypogaea h2 (Ara h2). Differential epitope responses were achieved by establishing a binding environment that minimized cross-reactivity while maximizing analytical sensitivity. IgE antibody binding to each Ara h2 epitope was distinguished and quantified from patient serum samples (10 μL each) in a 45 min assay. Excellent correlation of Ara h2-specific IgE values was found between ImmunoCAP assays and the new SPRi method.

Indexed as

Surface Plasmon Resonance2S Albumins, PlantAntigen-Antibody ReactionsAntigens, PlantArachisEpitopesGlycoproteinsHumansImmunoglobulin E2S Albumins, PlantAntigens, PlantAra h 2 allergen, Arachis hypogaeaEpitopesGlycoproteinsImmunoglobulin Eimmunoassaysimmunoglobulin Emagnetic beadpeanut allergysurface plasmon resonance (SPR) imaging

Identifiers

PMID29232483
PMCPMC5965296
OpenAlexW2774623446

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.