ArticlePloS one2017
Improved sample treatment protocol for accurate detection of live Salmonella spp. in food samples by viability PCR.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Optimization of the viability PCR for accurate detection of Staphylococcus aureus in food samples.PloS one · 2025Article
- A Review of Modern Methods for the Detection of Foodborne Pathogens.Microorganisms · 2023Review
- An Improved Real-Time Viability PCR Assay to DetectInternational journal of molecular sciences · 2022Article
- Screening and identification of BP100 peptide conjugates active against Xylella fastidiosa using a viability-qPCR method.BMC microbiology · 2020Article
- Key Factors for Removing Bias in Viability PCR-Based Methods: A Review.Current microbiology · 2020Review
- Spiking a Silty-Sand Reference Soil with Bacterial DNA: Limits and Pitfalls in the Discrimination of Live and Dead Cells When Applying Ethidium Monoazide (EMA) Treatment.Current microbiology · 2019Article
- Detection ofFoods (Basel, Switzerland) · 2019Review
- Monitoring Viable Cells of the Biological Control Agent Lactobacillus plantarum PM411 in Aerial Plant Surfaces by Means of a Strain-Specific Viability Quantitative PCR Method.Applied and environmental microbiology · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Culture-based detection is still considered as the standard way for detection of Salmonella in foods, although molecular methods, such as viability PCR (vPCR), have been introduced to overcome some disadvantages of traditional culture methods. Despite the success of the vPCR methodology, the problem of false-positive results is a major drawback, especially when applied to environmental samples, hindering the interpretation of the results. To improve the efficiency of vPCR, many approaches have been introduced by several authors during the last years. In the present work, the combination of PEMAX dye, double tube change, and double photo-activation step was established as a strategy to improve vPCR protocol. By combining these approaches, we developed an improved sample treatment protocol able to neutralize DNA signals of up to 5.0×107 dead cells/sample from both pure culture and artificially contaminated food samples. Our results indicate that vPCR can work reliable and has a potential for high throughput detection of live Salmonella cells in food samples, minimizing false-positive signals.
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Registered trials
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