Evidence map›Paper›PMID 29230626›Full record

ArticleMolecular biotechnology2018

Efficient One-Step Fusion PCR Based on Dual-Asymmetric Primers and Two-Step Annealing.

Yilan Liu, Jinjin Chen, Anders Thygesen

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Molecular biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 3 countries.

Yilan LiuDepartment of Chemical and Biomolecular Engineering, University of Nebraska Lincoln, Lincoln, NE, 68588, USA. liuyilan11@163.com.
Jinjin ChenState Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
Anders ThygesenCenter of Bioprocess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800, Lyngby, Denmark.
University of Nebraska–Lincoln · USTechnical University of Denmark · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gene splicing by fusion PCR is a versatile and widely used methodology, especially in synthetic biology. We here describe a rapid method for splicing two fragments by one-round fusion PCR with a dual-asymmetric primers and two-step annealing (ODT) method. During the process, the asymmetric intermediate fragments were generated in the early stage. Thereafter, they were hybridized in the subsequent cycles to serve as template for the target full-length product. The process parameters such as primer ratio, elongation temperature and cycle numbers were optimized. In addition, the fusion products produced with this method were successfully applied in seamless genome editing. The fusion of two fragments by this method takes less than 0.5 day. The method is expected to facilitate various kinds of complex genetic engineering projects with enhanced efficiency.

Indexed as

Cloning, MolecularDNA PrimersEscherichia coliGene EditingGene ExpressionNucleic Acid HybridizationPlasmidsPolymerase Chain ReactionRecombinant Fusion ProteinsTemperatureDNA PrimersRecombinant Fusion ProteinsDual-asymmetric primersFusion PCRTwo-step annealing

Identifiers

PMID29230626
OpenAlexW2773251497

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.