ArticleScientific reports2017
Three-dimensional (3D) culture of adult murine colon as an in vitro model of cryptosporidiosis: Proof of concept.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 35 citations in OpenAlex.
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- The application of explants, crypts, and organoids as models in intestinal barrier research.Tissue barriers · 2025Article
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- Microphysiological gut-on-chip enables extendedFrontiers in cellular and infection microbiology · 2025Article
- The Parasitism and Tumors Carcinogenesis: A Review Subject.Acta parasitologica · 2024Review
- Genomic and virulence analysis of in vitro cultured Cryptosporidium parvum.PLoS pathogens · 2024Article
- A review on the evolution of methods for intestinalVeterinary world · 2023Review
- ExploringFrontiers in cellular and infection microbiology · 2023Review
- Novel systems to study vector-pathogen interactions in malaria.Frontiers in cellular and infection microbiology · 2023Review
- An update onJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
- Transcending Dimensions in Apicomplexan Research: from Two-Dimensional to Three-DimensionalMicrobiology and molecular biology reviews : MMBR · 2022Review
- IsFood and waterborne parasitology · 2022Article
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- PersistentMicroorganisms · 2021Article
- Breakthroughs in microbiology made possible with organoids.PLoS pathogens · 2021Article
- The Truman Show for protozoan parasites: A review of in vitro cultivation platforms.PLoS neglected tropical diseases · 2021Review
- Refining Host-Pathogen Interactions: Organ-on-Chip Side of the Coin.Pathogens (Basel, Switzerland) · 2021Review
- The Stem Cell Revolution Revealing Protozoan Parasites' Secrets and Paving the Way towards Vaccine Development.Vaccines · 2021Review
Corrections and comments
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Authors and funding
16 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptosporidium parvum is a major cause of diarrheal illness and was recently potentially associated with digestive carcinogenesis. Despite its impact on human health, Cryptosporidium pathogenesis remains poorly known, mainly due to the lack of a long-term culture method for this parasite. Thus, the aim of the present study was to develop a three-dimensional (3D) culture model from adult murine colon allowing biological investigations of the host-parasite interactions in an in vivo-like environment and, in particular, the development of parasite-induced neoplasia. Colonic explants were cultured and preserved ex vivo for 35 days and co-culturing was performed with C. parvum. Strikingly, the resulting system allowed the reproduction of neoplastic lesions in vitro at 27 days post-infection (PI), providing new evidence of the role of the parasite in the induction of carcinogenesis. This promising model could facilitate the study of host-pathogen interactions and the investigation of the process involved in Cryptosporidium-induced cell transformation.
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Registered trials
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