ArticleScientific reports2017
Negative charge and membrane-tethered viral 3B cooperate to recruit viral RNA dependent RNA polymerase 3D
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Sequences and Structures of Viral Proteins Linked to the Genomes (VPg) of RNA Viruses.Viruses · 2025Review
- Inositol metabolism as a broad-spectrum antiviral target.Frontiers in microbiology · 2025Review
- In vitro reconstitution reveals membrane clustering and RNA recruitment by the enteroviral AAA+ ATPase 2C.PLoS pathogens · 2024Article
- Multiple functions of the nonstructural protein 3D in picornavirus infection.Frontiers in immunology · 2024Review
- Structure of monkeypox virus poxin: implications for drug design.Archives of virology · 2023Article
- A Helquat-like Compound as a Potent Inhibitor of Flaviviral and Coronaviral Polymerases.Molecules (Basel, Switzerland) · 2022Article
- Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit.Viruses · 2021Article
- Coxsackievirus B3 Exploits the Ubiquitin-Proteasome System to Facilitate Viral Replication.Viruses · 2021Article
- Osh6 Revisited: Control of PS Transport by the Concerted Actions of PI4P and Sac1 Phosphatase.Frontiers in molecular biosciences · 2021Article
- Structural analysis of the putative SARS-CoV-2 primase complex.Journal of structural biology · 2020Article
- Membrane Protein Dimerization in Cell-Derived Lipid Membranes Measured by FRET with MC Simulations.Biophysical journal · 2020Article
- Emerging Role for Acyl-CoA Binding Domain Containing 3 at Membrane Contact Sites During Viral Infection.Frontiers in microbiology · 2020Review
- Structural insights into Acyl-coenzyme A binding domain containing 3 (ACBD3) protein hijacking by picornaviruses.Protein science : a publication of the Protein Society · 2019Article
- Convergent evolution in the mechanisms of ACBD3 recruitment to picornavirus replication sites.PLoS pathogens · 2019Article
- The structural model of Zika virus RNA-dependent RNA polymerase in complex with RNA for rational design of novel nucleotide inhibitors.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most single stranded plus RNA viruses hijack phosphatidylinositol 4-kinases (PI4Ks) to generate membranes highly enriched in phosphatidylinositol 4-phosphate (PI4P). These membranous compartments known as webs, replication factories or replication organelles are essential for viral replication because they provide protection from the innate intracellular immune response while serving as platforms for viral replication. Using purified recombinant proteins and biomimetic model membranes we show that the nonstructural viral 3A protein is sufficient to promote membrane hyper-phosphorylation given the proper intracellular cofactors (PI4KB and ACBD3). However, our bio-mimetic in vitro reconstitution assay revealed that rather than the presence of PI4P specifically, negative charge alone is sufficient for the recruitment of 3D
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.