ReviewCell death and differentiation2018
Guidelines on experimental methods to assess mitochondrial dysfunction in cellular models of neurodegenerative diseases.
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Review in Cell death and differentiation, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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105citing papers in PubMed
4.7field-weighted citation impact, top 4% of its field
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Who cites it
105 citing papers in PubMed, 201 citations in OpenAlex.
- Astrocytes regulate axonal mitochondrial transport deficits in C9ORF72 amyotrophic lateral sclerosis motor neurons.Nature neuroscience · 2026Article
- Rhynchophylline rewires DLAT lipoylationActa pharmaceutica Sinica. B · 2026Article
- PFKM Modulates Porcine Skeletal Muscle Satellite Cell Differentiation Through Metabolic and Mitochondrial Pathways.Veterinary sciences · 2026Article
- Extracellular flux analyses indicate low ATP yield and require refinement for accurate determination of maximal oxygen consumption rate.Scientific reports · 2026Article
- Fluorescence-based quantification of mitochondrial damage in human airway smooth muscle cells.American journal of physiology. Cell physiology · 2026Article
- Mitochondrial dynamics in neurodevelopment and neurodevelopmental disorders.Nature reviews. Neuroscience · 2026Review
- Recapitulation of plaque formation, tau pathology, and neurodegeneration in a human 3D matrix model of Alzheimer's disease.Cell reports methods · 2026Article
- Mitochondrial quantity-quality imbalance in cellular senescence: practical readouts and minimal assay bundles.BMB reports · 2026Review
- Role of mitochondrial dysfunction in muscle wasting in cancer cachexia: a narrative review.Journal of translational medicine · 2026Review
- Loss of function variants in HPDL impair human cortical development via alterations of mitochondrial function.Cell death & disease · 2026Article
- Mitochondrial Dysfunction as a Targetable Vulnerability in Gastric Cancer: From Carcinogenesis to Apoptosis (A Narrative Review).Journal of Cancer · 2026Review
- Biomedical optical imaging of acne pathology: from single-modality methods to multimodal integration.Frontiers in medicine · 2026Review
- Pharmacological investigation of oxadiazole derivatives in Alzheimer's disease: Modulation of oxidative stress, neuroinflammation, and iNOS signaling.Iranian journal of basic medical sciences · 2026Article
- Integrating simulated and experimental data to identify mitochondrial bioenergetic defects in Parkinson's Disease models.PloS one · 2026Article
- Development of Cellular Energy Metabolism During Differentiation of Human iPSCs into Cortical Neurons.Molecular neurobiology · 2025Article
- Visualizing the internalization and biological impact of nanoplastics in live intestinal organoids by Fluorescence Lifetime Imaging Microscopy (FLIM).Light, science & applications · 2025Article
- Polyalanine Expansion in PABPN1 Alters the Structure and Dynamics of Its Nuclear Aggregates in Differentiated Muscle Cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- LDL Exposure Disrupts Mitochondrial Function and Dynamics in a Hippocampal Neuronal Cell Line.Molecular neurobiology · 2025Article
- Genetically encoded biosensors of metabolic function for the study of neurodegeneration, a review and perspective.Neurophotonics · 2025Review
- Mitochondrial Glutamine Metabolism Drives Epileptogenesis in Primary Hippocampal Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
45 more citing papers are in PubMed but not listed here.
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Authors and funding
46 authors at 20 institutions in 10 countries.
Niamh M C ConnollyDepartment of Physiology & Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.ORCID http://orcid.org/0000-0002-6005-1307
Pierre TheureyDepartment of Biomedical Sciences, University of Padova, Padova, Italy.
Vera Adam-ViziDepartment of Medical Biochemistry, Semmelweis University, Budapest, Hungary.
Nicolas G BazanNeuroscience Center of Excellence, School of Medicine, Louisiana State University Health New Orleans, New Orleans, LA, 70112, USA.
Paolo BernardiDepartment of Biomedical Sciences, University of Padova, Padova, Italy.ORCID http://orcid.org/0000-0001-9187-3736
Juan P BolañosUniversity of Salamanca, CIBERFES, Institute of Functional Biology and Genomics (IBFG), CSIC, 37007, Salamanca, Spain.
Carsten CulmseeInstitute of Pharmacology and Clinical Pharmacy, University of Marburg, 35043, Marburg, Germany.ORCID http://orcid.org/0000-0002-5121-5015
Valina L DawsonNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.ORCID http://orcid.org/0000-0002-2915-3970
Mohanish DeshmukhNeuroscience Center, University of North Carolina, Chapel Hill, USA.
Michael R DuchenDepartment of Cell and Developmental Biology and Consortium for Mitochondrial Research, University College London, London, WC1E 6BT, UK.ORCID http://orcid.org/0000-0003-2548-4294
Heiko DüssmannDepartment of Physiology & Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.
Gary FiskumDepartment of Anesthesiology and Center for Shock, Trauma and Anesthesiology Research (STAR), University of Maryland School of Medicine, Baltimore, MD, 21201, USA.
Maria F GalindoUnidad de Neuropsicofarmacología Translacional, Complejo Hospitalario Universitario de Albacete, Albacete, Spain.
Giles E HardinghamUK Dementia Research Institute at the University of Edinburgh, Edinburgh Medical School, Edinburgh, EH8 9XD, UK.
J Marie HardwickDepartment of Molecular Microbiology and Immunology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, MD, 21205, USA.
Mika B JekabsonsDepartment of Biology, University of Mississippi, University, MS, 38677, USA.
Elizabeth A JonasDepartment of Internal Medicine, Section of Endocrinology, Yale University, New Haven, CT, USA.
Joaquin JordánDepartment of Medical Science-Pharmacology, University of Castilla-La Mancha, Albacete, Spain.
Stuart A LiptonNeuroscience Translational Center and Depts. of Molecular Medicine and Neuroscience, The Scripps Research Institute, La Jolla, CA, 92037, USA.
Giovanni ManfrediBrain and Mind Research Institute, Weill Cornell Medicine, New York, NY, 10065, USA.
Mark P MattsonNational Institute on Aging Intramural Research Program, Baltimore, MD, USA.
BethAnn McLaughlinDepartment of Neurology, Vanderbilt University School of Medicine, Nashville, TN, USA.
Axel MethnerUniversity Medical Center Mainz, Dept. of Neurology, Mainz, Germany.ORCID http://orcid.org/0000-0002-8774-0057
Anne N MurphyDepartment of Pharmacology, University of California, San Diego, CA, 92093, USA.
Michael P MurphyMRC Mitochondrial Biology Unit, University of Cambridge, Cambridge Biomedical Campus, Cambridge, CB2 0XY, UK.
David G NichollsBuck Institute for Research on Aging, Novato, CA, 94945, USA.
Brian M PolsterDepartment of Anesthesiology and Center for Shock, Trauma and Anesthesiology Research (STAR), University of Maryland School of Medicine, Baltimore, MD, 21201, USA.ORCID http://orcid.org/0000-0002-8705-3628
Tullio PozzanDepartment of Biomedical Sciences, University of Padova, Padova, Italy.
Rosario RizzutoDepartment of Biomedical Sciences, University of Padova, Padova, Italy.
Jorgina SatrústeguiDpto. de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, 28049, Madrid, Spain.
Ruth S SlackBrain & Mind Research Institute, University of Ottawa, Ontario, K1H 8M5, Canada.
Raymond A SwansonDepartment of Neurology, University of California San Francisco and San Francisco Veterans Affairs Medical Center, San Francisco, CA, 94121, USA.
Russell H SwerdlowThe University of Kansas Alzheimer's Disease Center and Depts. of Neurology, Molecular and Integrative Physiology, and Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS, 66160, USA.
Yvonne WillPfizer, Groton, CT, 06340, USA.
Zheng YingLaboratory of Cellular and Molecular Neuropharmacology, Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, 215021, China.
Alvin JoselinBrain & Mind Research Institute, University of Ottawa, Ontario, K1H 8M5, Canada.
Anna GioranGerman Center for Neurodegenerative Diseases (DZNE), Bonn, Germany.
Catarina Moreira PinhoCenter for Alzheimer Research, Division of Neurogeriatrics, Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, Stockholm, Sweden.
Orla WattersDepartment of Physiology & Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.
Manuela SalvucciDepartment of Physiology & Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.
Irene Llorente-FolchDepartment of Physiology & Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.
David S ParkBrain & Mind Research Institute, University of Ottawa, Ontario, K1H 8M5, Canada.ORCID http://orcid.org/0000-0002-4490-3784
Daniele BanoGerman Center for Neurodegenerative Diseases (DZNE), Bonn, Germany.
Maria AnkarcronaCenter for Alzheimer Research, Division of Neurogeriatrics, Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, Stockholm, Sweden.
Paola PizzoDepartment of Biomedical Sciences, University of Padova, Padova, Italy.
Jochen H M PrehnDepartment of Physiology & Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland. prehn@rcsi.ie.
Royal College of Surgeons in Ireland · IEUniversity of Padua · ITUniversity of Ottawa · CAGerman Center for Neurodegenerative Diseases · DEJohns Hopkins University · USKarolinska Institutet · SEUniversity of Maryland, Baltimore · USBuck Institute for Research on Aging · USComplejo Hospitalario Universitario de Albacete · ESConsejo Superior de Investigaciones Científicas · ESCornell University · USJohannes Gutenberg University Mainz · DELouisiana State University Health Sciences Center New Orleans · USNational Institute on Aging · USPfizer (United States) · USPhilipps University of Marburg · DESan Francisco VA Medical Center · USScripps Research Institute · USSemmelweis University · HUSoochow University · CN
Funding
University of Kansas Alzheimer's Disease Core CentersP30AG035982 · NIA · UNIVERSITY OF KANSAS MEDICAL CENTER · PI SWERDLOW, RUSSELL H. · 2011 to 2020
$16.6MNovel Proteomics Approach to HIV-Associated Neurocognitive Disorder & Drug AbuseDP1DA041722 · NIDA · SCRIPPS RESEARCH INSTITUTE, THE · PI LIPTON, STUART A · 2016 to 2020
$7.0MNeuroprotective And Neurorestorative Signaling Mechanisms in Alzheimer's DiseaseZIAAG000314 · NIA · NATIONAL INSTITUTE ON AGING · PI MATTSON, MARK · 2009 to 2018
$6.7MCellular And Molecular Pathogenesis Of Alzheimer's DiseaseZIAAG000312 · NIA · NATIONAL INSTITUTE ON AGING · PI MATTSON, MARK · 2009 to 2018
$6.4MDynamic Interactions of the S-Nitrosoproteome in Type 2 Diabetes/Metabolic Syndrome and Alzheimer’s DiseaseRF1AG057409 · NIA · SCRIPPS RESEARCH INSTITUTE, THE · PI LIPTON, STUART A · 2017 to 2020
$4.1MS-Nitrosylation-Induced Posttranslational Modification and Aberrant Cell Signaling in Sporadic Alzheimer's DiseaseR01AG056259 · NIA · SCRIPPS RESEARCH INSTITUTE, THE · PI LIPTON, STUART A · 2017 to 2021
$3.3MModeling Parkinson's Disease with Isogenic hiPSC-Derived Dopaminergic NeuronsR01NS086890 · NINDS · SCRIPPS RESEARCH INSTITUTE, THE · PI LIPTON, STUART A · 2014 to 2018
$2.1MRegulatory Control of Glutamate - Induced Superoxide Production [Admin Supplement]R01NS081149 · NINDS · NORTHERN CALIFORNIA INSTITUTE/RES/EDU · PI SWANSON, RAYMOND A · 2012 to 2016
$1.7MThiol repletion therapy for Parkinson's diseaseI01BX003249 · VA · VETERANS AFFAIRS MED CTR SAN FRANCISCO · PI SWANSON, RAYMOND A · 2017 to 2020
–Biotechnology and Biological Sciences Research Council BB/L020874/1BLRD VA I01 BX003249CIHRMedical Research Council G0902044Medical Research Council MC_UU_00015/3NIA NIH HHS P30 AG035982NIA NIH HHS R01 AG056259NIA NIH HHS RF1 AG057409NIDA NIH HHS DP1 DA041722NINDS NIH HHS R01 NS081149NINDS NIH HHS R01 NS086890
Abstract
Neurodegenerative diseases are a spectrum of chronic, debilitating disorders characterised by the progressive degeneration and death of neurons. Mitochondrial dysfunction has been implicated in most neurodegenerative diseases, but in many instances it is unclear whether such dysfunction is a cause or an effect of the underlying pathology, and whether it represents a viable therapeutic target. It is therefore imperative to utilise and optimise cellular models and experimental techniques appropriate to determine the contribution of mitochondrial dysfunction to neurodegenerative disease phenotypes. In this consensus article, we collate details on and discuss pitfalls of existing experimental approaches to assess mitochondrial function in in vitro cellular models of neurodegenerative diseases, including specific protocols for the measurement of oxygen consumption rate in primary neuron cultures, and single-neuron, time-lapse fluorescence imaging of the mitochondrial membrane potential and mitochondrial NAD(P)H. As part of the Cellular Bioenergetics of Neurodegenerative Diseases (CeBioND) consortium ( www.cebiond.org ), we are performing cross-disease analyses to identify common and distinct molecular mechanisms involved in mitochondrial bioenergetic dysfunction in cellular models of Alzheimer's, Parkinson's, and Huntington's diseases. Here we provide detailed guidelines and protocols as standardised across the five collaborating laboratories of the CeBioND consortium, with additional contributions from other experts in the field.
Indexed as
Models, BiologicalAnimalsHumansMitochondriaNeurodegenerative Diseases
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