ArticleScientific reports2017
Transcriptome and metabolome profiling of Narcissus pseudonarcissus 'King Alfred' reveal components of Amaryllidaceae alkaloid metabolism.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 54 citations in OpenAlex.
- Silver Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Low-Molecular-Weight Compounds in a Narcissus Bulb.Molecules (Basel, Switzerland) · 2026Article
- Amaryllidaceae Alkaloids and Isoquinoline Alkaloids: A Perspective on Historical Approaches to Pathway Elucidation.Plants (Basel, Switzerland) · 2026Review
- Amaryllidaceae Alkaloids and Phenolic Acids Identification inMolecules (Basel, Switzerland) · 2026Article
- Elucidating the enzyme network driving Amaryllidaceae alkaloids biosynthesis in Leucojum aestivum.Plant biotechnology journal · 2025Article
- Effect of Auxins on the Accumulation of Alkaloids in UngraftedMolecules (Basel, Switzerland) · 2025Article
- Navigating Amaryllidaceae alkaloids: bridging gaps and charting biosynthetic territories.Journal of experimental botany · 2025Review
- Integrated metabolite profiling and transcriptome analysis identify candidate genes involved in diterpenoid alkaloid biosynthesis inFrontiers in plant science · 2025Article
- A developmental gradient reveals biosynthetic pathways to eukaryotic toxins in monocot geophytes.Cell · 2024Article
- Comprehensive Analysis of Metabolome and Transcriptome in Fruits and Roots of Kiwifruit.International journal of molecular sciences · 2023Article
- Characterization of norbelladine synthase and noroxomaritidine/norcraugsodine reductase reveals a novel catalytic route for the biosynthesis of Amaryllidaceae alkaloids including the Alzheimer's drug galanthamine.Frontiers in plant science · 2023Article
- Transcriptomic and co-expression network analyses on diverse wheat landraces identifies candidate master regulators of the response to early drought.Frontiers in plant science · 2023Article
- Comparative metabolomics analysis reveals alkaloid repertoires in young and mature Mitragyna speciosa (Korth.) Havil. Leaves.PloS one · 2023Article
- Comparative transcriptome and metabolome analyses identified the mode of sucrose degradation as a metabolic marker for early vegetative propagation in bulbs of Lycoris.The Plant journal : for cell and molecular biology · 2022Article
- Biotechnological Approaches to Optimize the Production of Amaryllidaceae Alkaloids.Biomolecules · 2022Review
- The ease and complexity of identifying and using specialized metabolites for crop engineering.Emerging topics in life sciences · 2022Review
- Transcriptome analysis of Leucojum aestivum and identification of genes involved in norbelladine biosynthesis.Planta · 2022Article
- Biological Investigation of Amaryllidaceae Alkaloid Extracts from the Bulbs ofMolecules (Basel, Switzerland) · 2021Article
- Revealing floral metabolite network in tuberose that underpins scent volatiles synthesis, storage and emission.Plant molecular biology · 2021Article
- Comprehensive Analysis of Transcriptome and Metabolome Reveals the Flavonoid Metabolic Pathway Is Associated with Fruit Peel Coloration of Melon.Molecules (Basel, Switzerland) · 2021Article
- Galantamine Quantity and Alkaloid Profile in the Bulbs ofMetabolites · 2021Article
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2 authors at 1 institution in 1 country.
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Abstract
Amaryllidaceae alkaloids (AAs) represent a diverse class of plant specialized metabolites and many display potent pharmacological activities. The AA metabolic pathway is poorly understood and resources are minimal. To enable AA pathway elucidation and novel biosynthetic enzymes discovery, we generated comprehensive metabolomic and corresponding transcriptomic datasets from different tissues of Narcissus pseudonarcissus 'King Alfred'. In this study, we performed untargeted UPLC-QTOF-MS metabolite analysis from different tissues, which generated exhaustive list of compounds, including several AAs, most predominant and diverse in bulbs. RNA sequencing of N. pseudonarcissus 'King Alfred' bulbs yielded 195,347 transcripts, after assembly. Top expressed genes belong to process like metabolism, survival, and defense including alkaloid biosynthetic genes. The transcriptome contained complete sequences for all proposed genes encoding AA-biosynthetic enzymes such as tyrosine decarboxylase (TYDC1 and TYDC2), phenylalanine ammonia-lyase (PAL1 and PAL2) and phenolic acids hydroxylases (C4H and C3H) to name a few. Furthermore, transcriptome data were validated using RT-qPCR analysis and expression study in different tissues of N. pseudonarcissus 'King Alfred' was performed. Here, we present the first comprehensive metabolome and transcriptome study from N. pseudonarcissus 'King Alfred' providing invaluable resources for metabolic engineering and biotechnological applications.
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