ArticleNature communications2017
Direction of actin flow dictates integrin LFA-1 orientation during leukocyte migration.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
72 citing papers in PubMed.
- Three cryo-EM structures of complement C3d-bound αScience advances · 2026Article
- 4polar3D single molecule imaging of 3D orientation in dense actin networks using ratiometric polarization splitting.Nature communications · 2026Article
- Review
- Rab4 spatially and functionally converges with Rab7 in the degradative endolysosomal network.Molecular biology of the cell · 2026Article
- Actin cortex formation is required for integrin clustering but not activation during leukocyte adhesion.International journal of biological sciences · 2026Article
- Interactions Between Active Matters and Endogenous Fields.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- TRPC4 regulates limbic behavior and neuronal development by stabilizing dendrite branches through actomyosin-driven integrin activation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Characterisation and correction of polarisation effects in fluorescently labelled fibres.Journal of microscopy · 2025Article
- TNF-α induces VE-cadherin-dependent gap/JAIL cycling through an intermediate state essential for neutrophil transmigration.Frontiers in immunology · 2025Article
- Dsg2 ectodomain organization increases throughout desmosome assembly.Cell adhesion & migration · 2024Article
- Illuminating cellular architecture and dynamics with fluorescence polarization microscopy.Journal of cell science · 2024Review
- Overview of the super-resolution and advanced microscopy imaging session at the 21st IUPAB and the 62nd Biophysics Society of Japan joint congress.Biophysical reviews · 2024Article
- Principles and regulation of mechanosensing.Journal of cell science · 2024Review
- OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images.PLoS computational biology · 2024Article
- Article
- Mechanical forces amplify TCR mechanotransduction in T cell activation and function.Applied physics reviews · 2024Review
- Piezo1 mechanosensing regulates integrin-dependent chemotactic migration in human T cells.eLife · 2024Article
- Can a bulky glycocalyx promote catch bonding in early integrin adhesion? Perhaps a bit.Biomechanics and modeling in mechanobiology · 2024Article
- Quantitative fluorescence emission anisotropy microscopy for implementing homo-fluorescence resonance energy transfer measurements in living cells.Molecular biology of the cell · 2023Article
- Review
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors.
Funding
Abstract
Integrin αβ heterodimer cell surface receptors mediate adhesive interactions that provide traction for cell migration. Here, we test whether the integrin, when engaged to an extracellular ligand and the cytoskeleton, adopts a specific orientation dictated by the direction of actin flow on the surface of migrating cells. We insert GFP into the rigid, ligand-binding head of the integrin, model with Rosetta the orientation of GFP and its transition dipole relative to the integrin head, and measure orientation with fluorescence polarization microscopy. Cytoskeleton and ligand-bound integrins orient in the same direction as retrograde actin flow with their cytoskeleton-binding β-subunits tilted by applied force. The measurements demonstrate that intracellular forces can orient cell surface integrins and support a molecular model of integrin activation by cytoskeletal force. Our results place atomic, Å-scale structures of cell surface receptors in the context of functional and cellular, μm-scale measurements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.