ArticleEndocrinology2018
Association of Gnrhr mRNA With the Stem Cell Determinant Musashi: A Mechanism for Leptin-Mediated Modulation of GnRHR Expression.
Article in Endocrinology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- ELAVL1 and ELAVL4 are required for Musashi-dependent translational activation.bioRxiv : the preprint server for biology · 2026Article
- Differential hypothalamic expression of Musashi protein 2 in Mongolian sheep with single and double ovulation.Frontiers in veterinary science · 2026Article
- Musashi-dependent mRNA translational activation is mediated through association with the Scd6/Like-sm family member, LSM14B.Scientific reports · 2025Article
- Ablation of Leptin Receptor Signaling Alters Somatotrope Transcriptome Maturation in Female Mice.Endocrinology · 2025Article
- Identification of FSH-regulated and estrous stage-specific transcriptional networks in mouse ovaries.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Case Report: Metreleptin treatment enables successful pregnancy in a female with congenital generalized lipodystrophy.Frontiers in endocrinology · 2025Article
- High fat diet-induced loss of pituitary plasticity in aging female mice with ablated leptin signaling in somatotropes.Frontiers in endocrinology · 2025Article
- The Musashi RNA binding proteins direct the translational activation of key pituitary mRNAs.Scientific reports · 2024Article
- Anterior Pituitary Transcriptomics Following a High-Fat Diet: Impact of Oxidative Stress on Cell Metabolism.Endocrinology · 2023Article
- Musashi Exerts Control of Gonadotrope Target mRNA Translation During the Mouse Estrous Cycle.Endocrinology · 2023Article
- Maternal undernutrition results in transcript changes in male offspring that may promote resistance to high fat diet induced weight gain.Frontiers in endocrinology · 2023Article
- Article
- Post-Transcriptional Regulation ofInternational journal of molecular sciences · 2021Review
- Control of the Anterior Pituitary Cell Lineage Regulator POU1F1 by the Stem Cell Determinant Musashi.Endocrinology · 2021Article
- The Importance of Leptin to Reproduction.Endocrinology · 2021Review
- Sex differences in somatotrope response to fasting: biphasic responses in male mice.The Journal of endocrinology · 2020Article
- Metabolic signalling to somatotrophs: Transcriptional and post-transcriptional mediators.Journal of neuroendocrinology · 2020Article
- Molecular Mechanisms of Pituitary Cell Plasticity.Frontiers in endocrinology · 2020Review
- Plasticity of Anterior Pituitary Gonadotrope Cells Facilitates the Pre-Ovulatory LH Surge.Frontiers in endocrinology · 2020Review
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
Abstract
The cyclic expression of pituitary gonadotropin-releasing hormone receptors (GnRHRs) may be an important checkpoint for leptin regulatory signals. Gonadotrope Lepr-null mice have reduced GnRHR levels, suggesting these receptors may be leptin targets. To determine if leptin stimulated GnRHR directly, primary pituitary cultures or pieces were exposed to 1 to 100 nM leptin. Leptin increased GnRHR protein levels and the percentages of gonadotropes that bound biotinylated analogs of gonadotropin-releasing hormone (bio-GnRH) but had no effect on Gnrhr messenger RNA (mRNA). An in silico analysis revealed three consensus Musashi (MSI) binding elements (MBEs) for this translational control protein in the 3' untranslated region (UTR) of Gnrhr mRNA. Several experiments determined that these Gnrhr mRNA MBE were active: (1) RNA electrophoretic mobility shift assay analyses showed that MSI1 specifically bound Gnrhr mRNA 3'-UTR; (2) RNA immunoprecipitation of pituitary fractions with MSI1 antibody pulled down a complex enriched in endogenous MSI protein and endogenous Gnrhr mRNA; and (3) fluorescence reporter assays showed that MSI1 repressed translation of the reporter coupled to the Gnrhr 3'-UTR. In vitro, leptin stimulation of pituitary pieces reduced Msi1 mRNA in female pituitaries, and leptin stimulation of pituitary cultures reduced MSI1 proteins selectively in gonadotropes identified by binding to bio-GnRH. These findings show that leptin's direct stimulatory actions on gonadotrope GnRHR correlate with a direct inhibition of expression of the posttranscriptional regulator MSI1. We also show MSI1 interaction with the 3'-UTR of Gnrhr mRNA. These findings now open the door to future studies of leptin-modulated posttranscriptional pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.