ArticlePLoS genetics2017
In vivo zebrafish morphogenesis shows Cyp26b1 promotes tendon condensation and musculoskeletal patterning in the embryonic jaw.
Article in PLoS genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 49 citations in OpenAlex.
- Trigeminal and facial branchiomotor neurons have shared muscle targets in zebrafish larvae.Journal of anatomy · 2026Article
- Hypoxia-activated scleraxis a mediates epicardial progenitor differentiation into a unique cardiac perivascular cell type.Nature communications · 2026Article
- Analysis of cranial tenocyte heterogeneity reveals a role for Wnt signaling in tendon attachments.Development (Cambridge, England) · 2026Article
- Gene regulatory programs underlying diversification of facial ligaments and tendons in zebrafish.Development (Cambridge, England) · 2026Article
- Dynamic cell fate plasticity and tissue reintegration drive functional adult synovial joint regeneration after complete resection.Nature communications · 2025Article
- Article
- The sclerotome is the source of the dorsal and anal fin skeleton and its expansion is required for median fin development.Development (Cambridge, England) · 2024Article
- The proteasome subunit psmb1 is essential for craniofacial cartilage maturation and morphogenesis.JCI insight · 2024Article
- Single-cell analysis reveals distinct fibroblast plasticity during tenocyte regeneration in zebrafish.Science advances · 2023Article
- Ligament injury in adult zebrafish triggers ECM remodeling and cell dedifferentiation for scar-free regeneration.NPJ Regenerative medicine · 2023Article
- Endogenous tenocyte activation underlies the regenerative capacity of the adult zebrafish tendon.NPJ Regenerative medicine · 2023Article
- Zebrafish crocc2 mutants exhibit divergent craniofacial shape, misregulated variability, and aberrant cartilage morphogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- A 1.1 Mb duplication CNV on chromosome 17 contributes to skeletal muscle development in Boer goats.Zoological research · 2023Article
- Ligament injury in adult zebrafish triggers ECM remodeling and cell dedifferentiation for scar-free regeneration.bioRxiv : the preprint server for biology · 2023Article
- Review
- Overview of Head Muscles with Special Emphasis on Extraocular Muscle Development.Advances in anatomy, embryology, and cell biology · 2023Article
- Embryonic ethanol exposure disrupts craniofacial neuromuscular integration in zebrafish larvae.Frontiers in physiology · 2023Article
- Coordinated patterning of zebrafish caudal fin symmetry by a central and two peripheral organizers.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Patterning of cartilaginous condensations in the developing facial skeleton.Developmental biology · 2022Article
- Hnrnpul1 controls transcription, splicing, and modulates skeletal and limb development in vivo.G3 (Bethesda, Md.) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Integrated development of diverse tissues gives rise to a functional, mobile vertebrate musculoskeletal system. However, the genetics and cellular interactions that drive the integration of muscle, tendon, and skeleton are poorly understood. In the vertebrate head, neural crest cells, from which cranial tendons derive, pattern developing muscles just as tendons have been shown to in limb and trunk tissue, yet the mechanisms of this patterning are unknown. From a forward genetic screen, we determined that cyp26b1 is critical for musculoskeletal integration in the ventral pharyngeal arches, particularly in the mandibulohyoid junction where first and second arch muscles interconnect. Using time-lapse confocal analyses, we detail musculoskeletal integration in wild-type and cyp26b1 mutant zebrafish. In wild-type fish, tenoblasts are present in apposition to elongating muscles and condense in discrete muscle attachment sites. In the absence of cyp26b1, tenoblasts are generated in normal numbers but fail to condense into nascent tendons within the ventral arches and, subsequently, muscles project into ectopic locales. These ectopic muscle fibers eventually associate with ectopic tendon marker expression. Genetic mosaic analysis demonstrates that neural crest cells require Cyp26b1 function for proper musculoskeletal development. Using an inhibitor, we find that Cyp26 function is required in a short time window that overlaps the dynamic window of tenoblast condensation. However, cyp26b1 expression is largely restricted to regions between tenoblast condensations during this time. Our results suggest that degradation of RA by this previously undescribed population of neural crest cells is critical to promote condensation of adjacent scxa-expressing tenoblasts and that these condensations are subsequently required for proper musculoskeletal integration.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.