ArticleBioorganic chemistry2018
Development of optimized conditions for Glaser-Hay bioconjugations.
Article in Bioorganic chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Development and Optimization of an Aminooxy Coupling Reaction to Prepare Multivalent Bioconjugates with a Single Noncanonical Amino Acid.Bioconjugate chemistry · 2026Article
- Hydrochalcogenation Reactions with Noncanonical Amino Acids as a Route to Increase Bioconjugate Valency.ACS omega · 2025Article
- Elucidation of Antimicrobials and Biofilm Inhibitors Derived from a Polyacetylene Core.Molecules (Basel, Switzerland) · 2024Article
- Effects of Structural Variations on Antibacterial Properties for Conjugated Diynes Generated through Glaser Hay Couplings.ChemMedChem · 2022Article
- Protein Modification Employing Non-Canonical Amino Acids to Prepare SUMOylation Detecting Bioconjugates.Pharmaceutics · 2022Article
- Genetic Encoding of a Bioconjugation Handle for [2+2+2] Cycloaddition Reactions.Chembiochem : a European journal of chemical biology · 2020Article
- A walk around the application of nanocatalysts for cross-dehydrogenative coupling of C-H bonds.RSC advances · 2019Review
- Mechanistic investigation and further optimization of the aqueous Glaser-Hay bioconjugation.Organic & biomolecular chemistry · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The efficient preparation of protein bioconjugates represents a route to novel materials, diagnostics, and therapeutics. We previously reported a novel bioorthogonal Glaser-Hay reaction for the preparation of covalent linkages between proteins and a reaction partner; however, deleterious protein degradation was observed under extended reaction conditions. Herein, we describe the systematic optimization of the reaction to increase coupling efficiency and decrease protein degradation. Two optimized conditions were identified varying either the pH of the reaction or the bidentate ligand employed, allowing for more rapid conjugations and/or less protein oxidation.
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Registered trials
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