ArticleProtein science : a publication of the Protein Society2018
Improved protein splicing using embedded split inteins.
Article in Protein science : a publication of the Protein Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Strategies and mechanisms of precision genome engineering: From gene editing to genome writing.iMetaOmics · 2026Review
- Click biology highlights the opportunities from reliable biological reactions.Nature chemical biology · 2025Review
- Protein editing using a coordinated transposition reaction.Science (New York, N.Y.) · 2025Article
- A cysteine-less and ultra-fast split intein rationally engineered from being aggregation-prone to highly efficient in protein trans-splicing.Nature communications · 2025Article
- Chemical Synthesis of Human Proteoforms and Application in Biomedicine.ACS central science · 2024Review
- Systemic delivery of full-length dystrophin in Duchenne muscular dystrophy mice.Nature communications · 2024Article
- Heterologous Production of Antimicrobial Peptides: Notes to Consider.The protein journal · 2024Article
- Simplified cloning and isolation of peptides from "sandwiched" SUMO-peptide-intein fusion proteins.BMC biotechnology · 2023Article
- Spatio-Temporal Photoactivation of Cytotoxic Proteins.Chembiochem : a European journal of chemical biology · 2022Article
- The Evolution of Intein-Based Affinity Methods as Reflected in 30 years of Patent History.Frontiers in molecular biosciences · 2022Review
- Live-cell protein engineering with an ultra-short split intein.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- An Atypical Mechanism of Split Intein Molecular Recognition and Folding.Journal of the American Chemical Society · 2018Article
- Efficient and wash-free labeling of membrane proteins using engineered Npu DnaE split-inteins.Protein science : a publication of the Protein Society · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Naturally split inteins mediate a traceless protein ligation process known as protein trans-splicing (PTS). Although frequently used in protein engineering applications, the efficiency of PTS can be reduced by the tendency of some split intein fusion constructs to aggregate; a consequence of the fragmented nature of the split intein itself or the polypeptide to which it is fused (the extein). Here, we report a strategy to help address this liability. This involves embedding the split intein within a protein sequence designed to stabilize either the intein fragment itself or the appended extein. We expect this approach to increase the scope of PTS-based protein engineering efforts.
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Registered trials
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