ArticleThe Journal of reproduction and development2018
Mancozeb impairs the ultrastructure of mouse granulosa cells in a dose-dependent manner.
Article in The Journal of reproduction and development, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Association between Female Reproductive Health and Mancozeb: Systematic Review of Experimental Models.International journal of environmental research and public health · 2020Pooled it
- Adverse Effects of Pesticides on the Ovary: Evidence from Epidemiological and Toxicological Studies.Environment & health (Washington, D.C.) · 2025Review
- Ultrastructural Changes of Neuroendocrine Pheochromocytoma Cell Line PC-12 Exposed In Vitro to Rotenone.Brain sciences · 2024Article
- Ultrastructural Analysis of Large Japanese Field Mouse (Biology · 2024Article
- How Do Environmental Toxicants Affect Oocyte Maturation Via Oxidative Stress?Advances in anatomy, embryology, and cell biology · 2024Review
- Ultrastructural Evaluation of Mouse Oocytes Exposed In Vitro to Different Concentrations of the Fungicide Mancozeb.Biology · 2023Article
- Deeply in Plasticenta: Presence of Microplastics in the Intracellular Compartment of Human Placentas.International journal of environmental research and public health · 2022Article
- Impairment of caprine oocyte maturation in vitro and alteration of granulosa cells functions by widely used fungicide mancozeb.Tropical animal health and production · 2021Article
- Endocrine-disrupting activity of mancozeb.Arhiv za farmaciju · 2021Article
- Female Fertility and Environmental Pollution.International journal of environmental research and public health · 2020Review
- Repeated hyperstimulation affects the ultrastructure of mouse fallopian tube epithelium.The Journal of reproduction and development · 2020Article
- Evaluation of Different Autologous Platelet Concentrate Biomaterials: Morphological and Biological Comparisons and Considerations.Materials (Basel, Switzerland) · 2020Article
- Pre-Implantation Mouse Embryos Cultured In Vitro under Different Oxygen Concentrations Show Altered Ultrastructures.International journal of environmental research and public health · 2020Article
- Commercial oral hygiene products and implant collar surfaces: Scanning electron microscopy observations.Canadian journal of dental hygiene : CJDH = Journal canadien de l'hygiene dentaire : JCHD · 2020Article
- Ultrastructural Assessment of the Anterolateral Ligament.Orthopaedic journal of sports medicine · 2019Article
- Preventative Effects of Vitamin E on Testicular Damage and Sperm Parameters in the First-Generation Mice Pups due to Pre- and Postnatal Mancozeb Exposure.Journal of toxicology · 2019Article
- Transcriptional landscape of mouse-aged ovaries reveals a unique set of non-coding RNAs associated with physiological and environmental ovarian dysfunctions.Cell death discovery · 2018Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mancozeb, an ethylene bis-dithiocarbamate, is widely used as a fungicide and exerts reproductive toxicity in vivo and in vitro in mouse oocytes by altering spindle morphology and impairing the ability to fertilize. Mancozeb also induces a premalignant status in mouse granulosa cells (GCs) cultured in vitro, as indicated by decreased p53 expression and tenuous oxidative stress. However, the presence and extent of ultrastructural alterations induced by mancozeb on GCs in vitro have not yet been reported. Using an in vitro model of reproductive toxicity, comprising parietal GCs from mouse antral follicles cultured with increasing concentrations of mancozeb (0.001-1 µg/ml), we sought to ascertain the in vitro ultrastructural cell toxicity by means of transmission (TEM) and scanning (SEM) electron microscopy. The results showed a dose-dependent toxicity of mancozeb on mouse GCs. Ultrastructural data showed intercellular contact alterations, nuclear membrane irregularities, and chromatin marginalization at lower concentrations, and showed chromatin condensation, membrane blebbing, and cytoplasmic vacuolization at higher concentrations. Morphometric analysis evidenced a reduction of mitochondrial length in GCs exposed to mancozeb 0.01-1 µg/ml and a dose-dependent increase of vacuole dimension. In conclusion, mancozeb induced dose-dependent toxicity against GCs in vitro, including ultrastructural signs of cell degeneration compatible with apoptosis, likely due to the toxic breakdown product ethylenethiourea. These alterations may represent a major cause of reduced/delayed/missed oocyte maturation in cases of infertility associated with exposure to pesticides.
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