Evidence map›Paper›PMID 29223047›Full record

ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2018

Identification and analysis of a mercapturic acid conjugate of indole-3-methyl isothiocyanate in the urine of humans who consumed cruciferous vegetables.

Pramod Upadhyaya, Adam T Zarth, Naomi Fujioka, Vincent A Fritz, Stephen S Hecht

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 86% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Pramod UpadhyayaMasonic Cancer Center, University of Minnesota, 2231 6th Street SE, Minneapolis, MN 55455, USA.
Adam T ZarthMasonic Cancer Center, University of Minnesota, 2231 6th Street SE, Minneapolis, MN 55455, USA.
Naomi FujiokaMasonic Cancer Center, University of Minnesota, 2231 6th Street SE, Minneapolis, MN 55455, USA.
Vincent A FritzSouthern Research and Outreach Center, University of Minnesota, 35838 120th Street, Waseca, MN 56093, USA.
Stephen S HechtMasonic Cancer Center, University of Minnesota, 2231 6th Street SE, Minneapolis, MN 55455, USA. Electronic address: hecht002@umn.edu.
University of Minnesota · USUniversity of Minnesota, Waseca · US

Funding

METABOLISM OF CARCINOGENIC TOBACCO SPECIFIC NITROSAMINESR01CA081301 · NCI · UNIVERSITY OF MINNESOTA TWIN CITIES · PI STEPHEN S HECHT · 1999 to 2026
$7.1M
Metabolism of Carcinogenic Tobacco-Specific NitrosaminesR37CA081301 · NCI · UNIVERSITY OF MINNESOTA TWIN CITIES · PI HECHT, STEPHEN S · 2004 to 2013
$5.5M
NCI NIH HHS R01 CA081301NCI NIH HHS R37 CA081301
6 · The paper itself

Abstract

Glucobrassicin, a quantitatively significant constituent of Brassica vegetables, gives rise to indole-3-carbinol (I3C) and its dimer di-indolylmethane (DIM) when the vegetables are chewed. I3C and DIM have been extensively studied with respect to their anti-carcinogenic properties. However, the presumed intermediate isothiocyanate in their formation, indole-3-methyl isothiocyanate (IMITC), has to our knowledge never been observed, despite the fact that isothiocyanates derived from cruciferous vegetables are known to have anti-carcinogenic properties. Therefore, we investigated the formation and presence in human urine of IMITC by analyzing for its N-acetylcysteine conjugate, IMITC-NAC, in order to gain a more complete understanding of the biochemical pathways leading to formation of I3C and DIM upon consumption of vegetables rich in glucobrassicin. Standard IMITC-NAC was synthesized and its structure confirmed by NMR and MS. IMITC-NAC was identified in extracts of Brussels sprouts chopped in the presence of N-acetylcysteine. An LC-ESI-MS/MS-SRM method for analysis of IMITC-NAC, with [

Indexed as

BrassicaVegetablesAcetylcysteineAdolescentAdultFemaleGlucosinolatesHumansIndolesIsocyanatesMaleMiddle AgedYoung AdultAcetylcysteineglucobrassicinGlucosinolatesIndolesIsocyanatesCruciferous vegetablesGlucobrassicinIndole-3-carbinolIndole-3-methyl isothiocayanate

Identifiers

PMID29223047
PMCPMC5743587
OpenAlexW2773266439

What OpenQuestion holds

Textmetadata
LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.