Genomic integration and expression of the Aggregatibacter actinomycetemcomitans catalase gene in Aggregatibacter aphrophilus.

Yuting Alice Yang, Ya-An Cheng, Casey Chen

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Article in Archives of oral biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 citing paper in PubMed.

The ArcAB two-component system is associated with the susceptibility ofmSphere · 2025
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Authors and funding

3 authors.

Yuting Alice YangDivision of Periodontology, Diagnostic Sciences and Dental Hygiene, Herman Ostrow School of Dentistry, University of Southern California, 925 W 34th street, Los Angeles, CA 90089, USA. Electronic address: yutingy@usc.edu.

Ya-An ChengDivision of Periodontology, Diagnostic Sciences and Dental Hygiene, Herman Ostrow School of Dentistry, University of Southern California, 925 W 34th street, Los Angeles, CA 90089, USA. Electronic address: yaanchen@gmail.com.

Casey ChenDivision of Periodontology, Diagnostic Sciences and Dental Hygiene, Herman Ostrow School of Dentistry, University of Southern California, 925 W 34th street, Los Angeles, CA 90089, USA. Electronic address: ccchen@usc.edu.

Funding

Novel clone-specific virulence determinants of A. actinomycetemcomitansR01DE012212 · NIDCR · UNIVERSITY OF SOUTHERN CALIFORNIA · PI CHEN, CASEY · 1999 to 2016

$5.0M

NIDCR NIH HHS R01 DE012212

6 · The paper itself

Abstract

objectiveTo test the hypothesis that virulence genes of Aggregatibacter actinomycetemcomitans can be expressed and confer fitness advantages in the closely related Aggregatibacter aphrophilus.

designClinical isolates of A. aphrophilus were screened for natural competence with marked genomic DNA from A. actinomycetemcomitans and A. aphrophilus. The gene katA of A. actinomycetemcomitans D7S-1 and its flanking regions were constructed and inserted into a comparable locus in the genome of a naturally competent A. aphrophilus strain by a markerless protocol via natural transformation. Mutants of A. actinomycetemcomitans with or without katA were also constructed by a similar protocol. Discs soaked with either 0.03% hydrogen peroxide or broth culture of Streptococcus gordonii Challis were placed on the agar with cultures of A. actinomycetemcomitans or A. aphrophilus. The size of the growth inhibition zone associated with the disc was measured after 2-day culture.

resultsFive of the 13A. aphrophilus strains exhibited a transformation frequency of 10

conclusionsThere was no apparent species barrier for the transfer and expression of A. actinomycetemcomitans katA in A. aphrophilus. The inserted A. actinomycetemcomitans-specific katA gene in A. aphrophilus strain NJ8700 conferred resistance to inhibition by hydrogen peroxide or S. gordonii. The potential to swap genes between these two closely related oral species may be an alternative approach for investigating the virulence determinants of A. actinomycetemcomitans.

Indexed as

Aggregatibacter actinomycetemcomitansAggregatibacter aphrophilusCatalaseGene Transfer TechniquesGenomic IslandsGenomicsHydrogen PeroxideMutagenesis, InsertionalPolymerase Chain ReactionVirulenceCatalaseHydrogen PeroxideCatalaseGene transferGenomic islandsHydrogen peroxideMutagenesisVirulence

Identifiers

PMID29223024

DOI10.1016/j.archoralbio.2017.12.002

PMCPMC5792192

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