Evidence map›Paper›PMID 29222109›Full record

ArticleJournal of cell science2018

MARCKS-related protein regulates cytoskeletal organization at cell-cell and cell-substrate contacts in epithelial cells.

Christina M Van Itallie, Amber Jean Tietgens, Angel Aponte, Marjan Gucek, Alexander X Cartagena-Rivera, Richard S Chadwick, James M Anderson

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
0.5field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Article
  4. Article
  5. Article
  6. Review
  7. MARCKS and Lung Disease.American journal of respiratory cell and molecular biology · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Christina M Van ItallieLaboratory of Tight Junction Structure and Function, National Institutes of Health, Building 50, Room 4525, 50 South Drive, Bethesda, MD 20892, USA Christina.vanitallie@nih.gov.ORCID 0000-0003-1687-731X
Amber Jean TietgensLaboratory of Tight Junction Structure and Function, National Institutes of Health, Building 50, Room 4525, 50 South Drive, Bethesda, MD 20892, USA.
Angel AponteProteomics Core, National Heart, Lung and Blood Institute, National Institutes of Health, Building 50, Room 4525, 50 South Drive, Bethesda, MD 20892, USA.
Marjan GucekProteomics Core, National Heart, Lung and Blood Institute, National Institutes of Health, Building 50, Room 4525, 50 South Drive, Bethesda, MD 20892, USA.
Alexander X Cartagena-RiveraSection on Auditory Mechanics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Building 50, Room 4525, 50 South Drive, Bethesda, MD 20892, USA.
Richard S ChadwickSection on Auditory Mechanics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Building 50, Room 4525, 50 South Drive, Bethesda, MD 20892, USA.
James M AndersonLaboratory of Tight Junction Structure and Function, National Institutes of Health, Building 50, Room 4525, 50 South Drive, Bethesda, MD 20892, USA.
National Institutes of Health · US

Funding

Proteomics CoreZICHL005903 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI GUCEK, MARJAN · 2009 to 2022
$23.3M
Biomechanics Of Cochlear Fine TuningZIADC000033 · NIDCD · NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS · PI CHADWICK, RICHARD S. · 2009 to 2019
$8.4M
Novel approaches to study dynamic coupling of the tight junction barrier to the actin cytoskeletonZIAHL006207 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI ANDERSON, JAMES · 2016 to 2016
$461k
Intramural NIH HHS ZIA HL006207
6 · The paper itself

Abstract

Treatment of epithelial cells with interferon-γ and TNF-α (IFN/TNF) results in increased paracellular permeability. To identify relevant proteins mediating barrier disruption, we performed proximity-dependent biotinylation (BioID) of occludin and found that tagging of MARCKS-related protein (MRP; also known as MARCKSL1) increased ∼20-fold following IFN/TNF administration. GFP-MRP was focused at the lateral cell membrane and its overexpression potentiated the physiological response of the tight junction barrier to cytokines. However, deletion of MRP did not abrogate the cytokine responses, suggesting that MRP is not required in the occludin-dependent IFN/TNF response. Instead, our results reveal a key role for MRP in epithelial cells in control of multiple actin-based structures, likely by regulation of integrin signaling. Changes in focal adhesion organization and basal actin stress fibers in MRP-knockout (KO) cells were reminiscent of those seen in FAK-KO cells. In addition, we found alterations in cell-cell interactions in MRP-KO cells associated with increased junctional tension, suggesting that MRP may play a role in focal adhesion-adherens junction cross talk. Together, our results are consistent with a key role for MRP in cytoskeletal organization of cell contacts in epithelial cells.

Indexed as

Adherens JunctionsAnimalsCell MovementCytokinesCytoskeletal ProteinsCytoskeletonDogsEpithelial CellsFocal AdhesionsGene Knockout TechniquesMadin Darby Canine Kidney CellsPeptide FragmentsTight Junction ProteinsTight JunctionsCytokinesCytoskeletal ProteinsMARCKS-related peptidePeptide FragmentsTight Junction ProteinsActinCell contactCytoskeletonFocal adhesionMarcksl1MRP

Identifiers

PMID29222109
PMCPMC5826046
OpenAlexW2771383741

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.