ArticleGenome biology and evolution2017
High-Resolution Single-Cell Sequencing of Malaria Parasites.
Article in Genome biology and evolution, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Replication stress increases de novo CNVs across the malaria parasite genome.Nucleic acids research · 2026Article
- Replication stress increases de novo CNVs across the malaria parasite genome.bioRxiv : the preprint server for biology · 2025Article
- Malaria: past, present, and future.Signal transduction and targeted therapy · 2025Review
- Article
- Bulk segregant linkage mapping for rodent and human malaria parasites.Parasitology international · 2022Review
- Whole Genome Sequencing Contributions and Challenges in Disease Reduction Focused on Malaria.Biology · 2022Review
- Genetic surveillance for monitoring the impact of drug use on Plasmodium falciparum populations.International journal for parasitology. Drugs and drug resistance · 2021Review
- Single-genome sequencing reveals within-host evolution of human malaria parasites.Cell host & microbe · 2021Article
- Single-cell genome sequencing of protozoan parasites.Trends in parasitology · 2021Review
- Single-cell sequencing of the small and AT-skewed genome of malaria parasites.Genome medicine · 2021Article
- Extrachromosomal DNA amplicons in antimalarial-resistant Plasmodium falciparum.Molecular microbiology · 2021Article
- Merozoite Proteins Discovered by qRT-PCR-Based Transcriptome Screening ofFrontiers in cellular and infection microbiology · 2021Article
- Article
- Co-transmission of Related Malaria Parasite Lineages Shapes Within-Host Parasite Diversity.Cell host & microbe · 2020Article
- Genome-wide microsatellite characteristics of five human Plasmodium species, focusing on Plasmodium malariae and P. ovale curtisi.Parasite (Paris, France) · 2020Article
- Advances in omics-based methods to identify novel targets for malaria and other parasitic protozoan infections.Genome medicine · 2019Review
- Review
- Applying next-generation sequencing to track falciparum malaria in sub-Saharan Africa.Malaria journal · 2019Review
- Article
- Discrimination Experiments in Entamoeba and Evidence from Other Protists Suggest Pathogenic Amebas Cooperate with Kin to Colonize Hosts and Deter Rivals.The Journal of eukaryotic microbiology · 2019Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Single-cell genomics is a powerful tool for determining the genetic architecture of complex communities of unicellular organisms. In areas of high transmission, malaria patients are often challenged by the activities of multiple Plasmodium falciparum lineages, which can potentiate pathology, spread drug resistance loci, and also complicate most genetic analysis. Single-cell sequencing of P. falciparum would be key to understanding infection complexity, though efforts are hampered by the extreme nucleotide composition of its genome (∼80% AT-rich). To counter the low coverage achieved in previous studies, we targeted DNA-rich late-stage parasites by Fluorescence-Activated Cell Sorting and whole genome sequencing. Our method routinely generates accurate, near-complete capture of the 23 Mb P. falciparum genome (mean breadth of coverage 90.7%) at high efficiency. Data from 48 single-cell genomes derived from a polyclonal infection sampled in Chikhwawa, Malawi allowed for unambiguous determination of haplotype diversity and recent meiotic events, information that will aid public health efforts.
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Registered trials
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