ArticlePloS one2017
Zebrafish skeleton development: High resolution micro-CT and FIB-SEM block surface serial imaging for phenotype identification.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Transcriptomic Profiling of Zebrafish Mutant forInternational journal of molecular sciences · 2025Article
- Specimen preparation for X-ray micro-computed tomography of forest pests.Applied microscopy · 2025Review
- Zebrafish Models to Study Ectopic Calcification and Calcium-Associated Pathologies.International journal of molecular sciences · 2023Review
- Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish.Cardiovascular research · 2022Article
- Article
- Dynamics of the Zebrafish Skeleton in Three Dimensions During Juvenile and Adult Development.Frontiers in physiology · 2022Article
- High resolution 3D structures of mineralized tissues in health and disease.Nature reviews. Endocrinology · 2021Review
- Parallel Reaction Monitoring reveals structure-specific ceramide alterations in the zebrafish.Scientific reports · 2019Article
- Using zebrafish to study skeletal genomics.Bone · 2019Review
- Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography.Journal of visualized experiments : JoVE · 2018Article
Corrections and comments
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Authors and funding
8 authors.
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Abstract
Although bone is one of the most studied living materials, many questions about the manner in which bones form remain unresolved, including fine details of the skeletal structure during development. In this study, we monitored skeleton development of zebrafish larvae, using calcein fluorescence, high-resolution micro-CT 3D images and FIB-SEM in the block surface serial imaging mode. We compared calcein staining of the skeletons of the wild type and nacre mutants, which are transparent zebrafish, with micro-CT for the first 30 days post fertilization embryos, and identified significant differences. We quantified the bone volumes and mineral contents of bones, including otoliths, during development, and showed that such developmental differences, including otolith development, could be helpful in identifying phenotypes. In addition, high-resolution imaging revealed the presence of mineralized aggregates in the notochord, before the formation of the first bone in the axial skeleton. These structures might play a role in the storage of the mineral. Our results highlight the potential of these high-resolution 3D approaches to characterize the zebrafish skeleton, which in turn could prove invaluable information for better understanding the development and the characterization of skeletal phenotypes.
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Registered trials
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