ArticleThe journal of physical chemistry letters2017
Electric Fields and Fast Protein Dynamics in Enzymes.
Article in The journal of physical chemistry letters, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Electric Fields in Polymeric Systems.Chemical reviews · 2024Review
- The Evolution of the Acylation Mechanism inACS catalysis · 2024Article
- Review
- Protein Dynamics and Enzymatic Catalysis.The journal of physical chemistry. B · 2023Review
- Connecting Conformational Motions to Rapid Dynamics in Human Purine Nucleoside Phosphorylase.The journal of physical chemistry. B · 2023Article
- Proton transfer reactions: From photochemistry to biochemistry and bioenergetics.BBA advances · 2023Article
- Perspective: Path Sampling Methods Applied to Enzymatic Catalysis.Journal of chemical theory and computation · 2022Review
- Boosting the performance of single-atom catalysts via external electric field polarization.Nature communications · 2022Article
- The Interplay of Electrostatics and Chemical Positioning in the Evolution of Antibiotic Resistance in TEM β-Lactamases.ACS central science · 2021Article
- Catalytic Fields as a Tool to Analyze Enzyme Reaction Mechanism Variants and Reaction Steps.The journal of physical chemistry. B · 2021Article
- Inverse heavy enzyme isotope effects in methylthioadenosine nucleosidases.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Local Electric Fields as a Natural Switch of Heme-Iron Protein Reactivity.ACS catalysis · 2021Article
- Role of Protein Motions in Catalysis by Formate Dehydrogenase.The journal of physical chemistry. B · 2020Article
- A Preorganized Electric Field Leads to Minimal Geometrical Reorientation in the Catalytic Reaction of Ketosteroid Isomerase.Journal of the American Chemical Society · 2020Article
- Extreme Catalytic Power of Ketosteroid Isomerase Related to the Reversal of Proton Dislocations in Hydrogen-Bond Network.The journal of physical chemistry. B · 2020Article
- A Critical Evaluation of Vibrational Stark Effect (VSE) Probes with the Local Vibrational Mode Theory.Sensors (Basel, Switzerland) · 2020Article
- Effect of Protein Isotope Labeling on the Catalytic Mechanism of Lactate Dehydrogenase.The journal of physical chemistry. B · 2019Article
- Examining the Origin of Catalytic Power of Catechol O-Methyltransferase.ACS catalysis · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In recent years, there has been much discussion regarding the origin of enzymatic catalysis and whether including protein dynamics is necessary for understanding catalytic enhancement. An important contribution in this debate was made with the application of the vibrational Stark effect spectroscopy to measure electric fields in the active site. This provided a window on electric fields at the transition state in enzymatic reactions. We performed computational studies on two enzymes where we have shown that fast dynamics is part of the reaction mechanism and calculated the electric field near the bond-breaking event. We found that the fast motions that we had identified lead to an increase of the electric field, thus preparing an enzymatic configuration that is electrostatically favorable for the catalytic chemical step. We also studied the enzyme that has been the subject of Stark spectroscopy, ketosteroid isomerase, and found electric fields of a similar magnitude to the two previous examples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.