ArticleCancer biology & therapy2018
Multispectral imaging reveals hyper active TGF-β signaling in colorectal cancer.
Article in Cancer biology & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- ADI-270: an armored allogeneic gamma delta T cell therapy designed to target CD70-expressing solid and hematologic malignancies.Journal for immunotherapy of cancer · 2025Article
- Predicting Survival Among Colorectal Cancer Patients: Development and Validation of Polygenic Survival Score.Clinical and experimental gastroenterology · 2024Article
- TGF-β in correlation with tumor progression, immunosuppression and targeted therapy in colorectal cancer.Medical oncology (Northwood, London, England) · 2023Review
- VPS9D1-AS1 overexpression amplifies intratumoral TGF-β signaling and promotes tumor cell escape from CD8eLife · 2022Article
- Regulation of transforming growth factor-β signaling as a therapeutic approach to treating colorectal cancer.World journal of gastroenterology · 2022Review
- Assessment of the TGFB1 gene expression and methylation status of the promoter region in patients with colorectal cancer.Scientific reports · 2022Article
- Article
- Correlation between Oxidative Stress and Transforming Growth Factor-Beta in Cancers.International journal of molecular sciences · 2021Review
- A pilot study of near-infrared fluorescence guided surgery for primary tumor localization and lymph node mapping in colorectal cancer.Annals of translational medicine · 2021Article
- Transforming Growth Factor-Cancers · 2021Review
- Current and Prospective Methods for Assessing Anti-Tumor Immunity in Colorectal Cancer.International journal of molecular sciences · 2021Review
- Impaired AGO2/miR-185-3p/NRP1 axis promotes colorectal cancer metastasis.Cell death & disease · 2021Article
- Phosphorylation of SMAD3 in immune cells predicts survival of patients with early stage non-small cell lung cancer.Journal for immunotherapy of cancer · 2021Article
- A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections.Journal of visualized experiments : JoVE · 2020Article
- Overexpression of NNT-AS1 Activates TGF-BioMed research international · 2020Article
- Multiplex staining depicts the immune infiltrate in colitis-induced colon cancer model.Scientific reports · 2019Article
- Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry.Journal of visualized experiments : JoVE · 2019Article
- Article
- Multiplex fluorescent immunohistochemistry quantitatively analyses microvascular density (MVD) and the roles of TGF-β signalling in orchestrating angiogenesis in colorectal cancer.Translational cancer research · 2019Article
- Analysis of the promoter region of the human miR-32 gene in colorectal cancer.Oncology letters · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in multiplex immunohistochemistry (IHC) techniques and digital pathology platforms allow quantification of multiple proteins at same tissue section and produce continuous data. TGF-β signaling plays crucial and complex roles in colorectal cancer (CRC). We here aimed to investigate clinical pathological relevant of proteins involved in TGF-β signaling at CRC tissues. Multiplex fluorescent IHC was used to quantitative analysis. The levels of eight proteins (TGF-β1, TGFBRI, TGFBRII, SMAD4, SMAD2/3, p-SMAD2/3, SMAD1/5/9, and p-SMAD1/5/9) were determined in TMA sections. Quantitative analysis was carried out by a scoring system by InForm software. It revealed that TGF-β signaling was hyper active. The levels of TGF-β1, TGFBRI, TGFBRII, SMAD4, SMAD1/5/9 and p-SMAD2/3 were significantly increased in cancer tissues when compared their levels in normal tissues. Furthermore, the levels of eight proteins in stroma were significantly lower than the levels that in cancer tissues. Clinical pathological relevant analysis exhibited that TGF-β signaling inclined to suppress the progression of tumor. SMAD1/5/9, TGFBRII, SMAD2/3 were confirmed as significant predictors for overall survival. In conclusion, we established a method based on multispectral imaging to extensively explore the clinical relevant of TGF-β signaling proteins. These results provided an opportunity to consider the novel application for proteins involving TGF-β signaling that used as diagnostic or prognostic biomarkers to conduct tumor therapy.
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Registered trials
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