Evidence map›Paper›PMID 29219110›Full record

ArticleParasite (Paris, France)2017

Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli.

Grégory Karadjian, Aurélie Heckmann, Giuseppe La Rosa, Edoardo Pozio, Pascal Boireau, Isabelle Vallée

Abstract read
In one paragraph

Article in Parasite (Paris, France), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Genetic identification ofFrontiers in veterinary science · 2023
    Article
  6. Article
  7. Article
  8. Review
  9. The First Report ofIranian journal of parasitology
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Grégory KaradjianJRU BIPAR, ANSES, École Nationale Vétérinaire d'Alfort, INRA, OIE Collaborating Centre for Foodborne Zoonotic Parasites, Laboratory for Animal Health, 14 Rue Pierre et Marie Curie, 94701 Maisons-Alfort Cedex, France.
Aurélie HeckmannJRU BIPAR, ANSES, École Nationale Vétérinaire d'Alfort, INRA, OIE Collaborating Centre for Foodborne Zoonotic Parasites, Laboratory for Animal Health, 14 Rue Pierre et Marie Curie, 94701 Maisons-Alfort Cedex, France.
Giuseppe La RosaEuropean Union Reference Laboratory for Parasites, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
Edoardo PozioEuropean Union Reference Laboratory for Parasites, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
Pascal BoireauJRU BIPAR, ANSES, École Nationale Vétérinaire d'Alfort, INRA, OIE Collaborating Centre for Foodborne Zoonotic Parasites, Laboratory for Animal Health, 14 Rue Pierre et Marie Curie, 94701 Maisons-Alfort Cedex, France.
Isabelle ValléeJRU BIPAR, ANSES, École Nationale Vétérinaire d'Alfort, INRA, OIE Collaborating Centre for Foodborne Zoonotic Parasites, Laboratory for Animal Health, 14 Rue Pierre et Marie Curie, 94701 Maisons-Alfort Cedex, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In order to identify Trichinella at the species level, the commonly used test is a multiplex PCR, allowing the discrimination of nine out of the twelve taxa described so far. This test is based on five primer pairs amplifying fragments of the large subunit rDNA. Each taxon produces one or two bands of different sizes, resulting in a specific band pattern. By multiplex PCR, Trichinella murrelli shows two bands of 127 bp and 316 bp. However, a third band of 256 bp can occur. This band can lead to misidentification, since it is similar to the 253 bp band displayed by Trichinella britovi. BLAST analysis confirmed that the 256 bp band is from T. murrelli. The aim of this short note is to inform analysts that T. murrelli larvae may display either two- or three-band patterns.

Indexed as

Multiplex Polymerase Chain ReactionAnimalsDNA, HelminthElectrophoresis, Agar GelFemaleLarvaMiceSequence AlignmentTrichinellaDNA, Helminth

Identifiers

PMID29219110
PMCPMC5721686

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.