ArticleBMC microbiology2017
6S RNA plays a role in recovery from nitrogen depletion in Synechocystis sp. PCC 6803.
Article in BMC microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Inorganic carbon levels regulate growth via SigC signaling cascade in cyanobacteria.The New phytologist · 2025Article
- The genome sequence of Synechocystis sp. PCC 6803 substrain GT-T and its implications for the evolution of PCC 6803 substrains.FEBS open bio · 2023Article
- Post-transcriptional control of bacterial nitrogen metabolism by regulatory noncoding RNAs.World journal of microbiology & biotechnology · 2022Review
- 6S RNA-Dependent Susceptibility to RNA Polymerase Inhibitors.Antimicrobial agents and chemotherapy · 2022Article
- Involvement ofInternational journal of molecular sciences · 2022Article
- Roles of Close Homologues SigB and SigD in Heat and High Light Acclimation of the CyanobacteriumLife (Basel, Switzerland) · 2022Article
- Genetic Responses of Metabolically ActiveMicroorganisms · 2021Article
- Analysis of a photosynthetic cyanobacterium rich in internal membrane systems via gradient profiling by sequencing (Grad-seq).The Plant cell · 2021Article
- 6S-2 RNA deletion in the undomesticatedRNA biology · 2021Article
- Similarities and differences between 6S RNAs from Bradyrhizobium japonicum and Sinorhizobium meliloti.Journal of microbiology (Seoul, Korea) · 2020Article
- Modifying the Cyanobacterial Metabolism as a Key to Efficient Biopolymer Production in Photosynthetic Microorganisms.International journal of molecular sciences · 2020Review
- 6S-1 RNA Contributes to Sporulation and Parasporal Crystal Formation inFrontiers in microbiology · 2020Article
- Inactivation of group 2 σ factors upregulates production of transcription and translation machineries in the cyanobacterium Synechocystis sp. PCC 6803.Scientific reports · 2018Article
- Widespread Antisense Transcription in Prokaryotes.Microbiology spectrum · 2018Review
- Small RNAs Involved in Regulation of Nitrogen Metabolism.Microbiology spectrum · 2018Review
- 6S RNA, a Global Regulator of Transcription.Microbiology spectrum · 2018Review
- Comparative Targeted Proteomics of the Central Metabolism and Photosystems in SigE Mutant Strains ofMolecules (Basel, Switzerland) · 2018Article
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundThe 6S RNA is a global transcriptional riboregulator, which is exceptionally widespread among most bacterial phyla. While its role is well-characterized in some heterotrophic bacteria, we subjected a cyanobacterial homolog to functional analysis, thereby extending the scope of 6S RNA action to the special challenges of photoautotrophic lifestyles.
resultsPhysiological characterization of a 6S RNA deletion strain (ΔssaA) demonstrates a delay in the recovery from nitrogen starvation. Significantly decelerated phycobilisome reassembly and glycogen degradation are accompanied with reduced photosynthetic activity compared to the wild type. Transcriptome profiling further revealed that predominantly genes encoding photosystem components, ATP synthase, phycobilisomes and ribosomal proteins were negatively affected in ΔssaA. In vivo pull-down studies of the RNA polymerase complex indicated that the presence of 6S RNA promotes the recruitment of the cyanobacterial housekeeping σ factor SigA, concurrently supporting dissociation of group 2 σ factors during recovery from nitrogen starvation.
conclusionsThe combination of genetic, physiological and biochemical studies reveals the homologue of 6S RNA as an integral part of the cellular response of Synechocystis sp. PCC 6803 to changing nitrogen availability. According to these results, 6S RNA supports a rapid acclimation to changing nitrogen supply by accelerating the switch from group 2 σ factors SigB, SigC and SigE to SigA-dependent transcription. We therefore introduce the cyanobacterial 6S RNA as a novel candidate regulator of RNA polymerase sigma factor recruitment in Synechocystis sp. PCC 6803. Further studies on mechanistic features of the postulated interaction should shed additional light on the complexity of transcriptional regulation in cyanobacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.