ArticleNucleic acids research2018
Binary control of enzymatic cleavage of DNA origami by structural antideterminants.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Global Quantitative Analysis of Ligation Reactions in Self-Assembled DNA Nanostructures at the Single-Nick Level.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Uncovering Design and Assembly Rules for mRNA-DNA Origami.Nano letters · 2026Article
- Functions and applications of enzymes in nucleic acid nanotechnology.Journal of nanobiotechnology · 2025Review
- Time-Dependent DNA Origami Denaturation by Guanidinium Chloride, Guanidinium Sulfate, and Guanidinium Thiocyanate.International journal of molecular sciences · 2022Article
- Integrating CRISPR/Cas systems with programmable DNA nanostructures for delivery and beyond.iScience · 2022Review
- Salting-Out of DNA Origami Nanostructures by Ammonium Sulfate.International journal of molecular sciences · 2022Article
- DNA origami nano-mechanics.Chemical Society reviews · 2021Review
- Unraveling the interaction between doxorubicin and DNA origami nanostructures for customizable chemotherapeutic drug release.Nucleic acids research · 2021Article
- Nuclease resistance of DNA nanostructures.Nature reviews. Chemistry · 2021Review
- Effect of DNA Origami Nanostructures on hIAPP Aggregation.Nanomaterials (Basel, Switzerland) · 2020Article
- Challenges and Perspectives of DNA Nanostructures in Biomedicine.Angewandte Chemie (International ed. in English) · 2020Review
- Robotic DNA Nanostructures.ACS synthetic biology · 2020Review
- Global and local mechanical properties control endonuclease reactivity of a DNA origami nanostructure.Nucleic acids research · 2020Article
- The Growing Development of DNA Nanostructures for Potential Healthcare-Related Applications.Advanced healthcare materials · 2019Review
- Structural stability of DNA origami nanostructures under application-specific conditions.Computational and structural biotechnology journal · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Controlling DNA nanostructure interaction with protein is essential in developing nanodevices with programmable function, reactivity, and stability for biological and medical applications. Here, we show that the sequence-specific action of restriction endonucleases towards sharp triangular or rectangular DNA origami exhibits a novel, binary 'on/off' behaviour, as canonical recognition sites are either essentially fully reactive, or strongly resistant to enzymatic cutting. Moreover, introduction of structural defects in the sharp triangle can activate an otherwise unreactive site, with a site-to-defect distance of ∼50 nm. We argue that site reactivity is dependent upon programmable, mechanical coupling in the two-dimensional DNA origami, with specific structural elements, including DNA nicks and branches proximal to the sites that can function as negative(anti) determinants of reactivity. Empirically modelling the constraints to DNA degrees of freedom associated with each recognition site in the sharp triangle can rationalize the pattern of suppressed reactivity towards nine restriction endonucleases, in substantial agreement with the experimental results. These results provide a basis for a predictive understanding of structure-reactivity correlates of specific DNA nanostructures, which will allow a better understanding of the behaviour of nucleic acids under nanoscale confinement, as well as in the rational design of functional nanodevices based on self-assembling nucleic acids.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.