Evidence map›Paper›PMID 29215865›Full record

ArticleACS chemical neuroscience2018

Identification of Fluorescent Small Molecule Compounds for Synaptic Labeling by Image-Based, High-Content Screening.

Matthew Dunn, Umed Boltaev, Anne Beskow, Sergey Pampou, Ronald Realubit, Torcato Meira, João Vaz Silva, Rose Reeb, Charles Karan, Steffen Jockusch and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS chemical neuroscience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 3 countries.

Matthew DunnDepartment of Chemistry , Columbia University , New York , New York 10027 , United States.
Umed BoltaevDepartment of Chemistry , Columbia University , New York , New York 10027 , United States.
Anne BeskowDepartment of Pathology and Cell Biology , Columbia University Medical Center , New York , New York 10032 , United States.
Sergey PampouColumbia Genome Center High-throughput Screening Facility , Columbia University Medical Center , New York , New York 10032 , United States.
Ronald RealubitColumbia Genome Center High-throughput Screening Facility , Columbia University Medical Center , New York , New York 10032 , United States.
Torcato MeiraDepartment of Neuroscience , Columbia University Medical Center , New York , New York 10032 , United States.
João Vaz SilvaDepartment of Pathology and Cell Biology , Columbia University Medical Center , New York , New York 10032 , United States.
Rose ReebDepartment of Pathology and Cell Biology , Columbia University Medical Center , New York , New York 10032 , United States.
Charles KaranColumbia Genome Center High-throughput Screening Facility , Columbia University Medical Center , New York , New York 10032 , United States.
Steffen JockuschDepartment of Chemistry , Columbia University , New York , New York 10027 , United States.ORCID 0000-0002-4592-5280
David SulzerDepartments of Psychiatry and Neurology , Columbia University Medical Center , New York , New York 10032 , United States.
Young Tae ChangDepartment of Chemistry , Pohang University of Science and Technology , Pohang , Gyeongsangbuk-do , Republic of Korea.ORCID 0000-0002-1927-3688
Dalibor SamesDepartment of Chemistry , Columbia University , New York , New York 10027 , United States.
Clarissa L WaitesDepartment of Pathology and Cell Biology , Columbia University Medical Center , New York , New York 10032 , United States.ORCID 0000-0003-4263-5576
Columbia University Irving Medical Center · USColumbia University · USPohang University of Science and Technology · KR

Funding

The Presynaptic Vesicle Cycle for the Slow Acting Neurotransmitter SecretionP01DA010154 · NIDA · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI EDWARDS, ROBERT H, KREITZER, ANATOL · 1995 to 2016
$14.7M
Development of Fluorescent False NeurotransmittersR01MH108186 · NIMH · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI SAMES, DALIBOR, SULZER, DAVID · 2015 to 2024
$5.8M
Uncovering the Roles of Ubiquitination and the ESCRT Pathway in Degradative Sorting of SV Proteins.R01NS080967 · NINDS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI WAITES, CLARISSA LEIGH · 2013 to 2023
$3.8M
High-throughput screening platform for discovery of fluorescent synaptic markersR21MH104803 · NIMH · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI WAITES, CLARISSA LEIGH · 2014 to 2015
$440k
NIDA NIH HHS P01 DA010154NIMH NIH HHS R01 MH108186NIMH NIH HHS R21 MH104803NINDS NIH HHS R01 NS080967
6 · The paper itself

Abstract

Few tools are available for noninvasive imaging of synapses in the living mammalian brain. Current paradigms require the use of genetically modified mice or viral delivery of genetic material to the brain. To develop an alternative chemical approach, utilizing the recognition of synaptic components by organic small molecules, we designed an imaging-based, high-content screen in cultured cortical neurons to identify molecules based on their colocalization with fluorescently tagged synaptic proteins. We used this approach to screen a library of ∼7000 novel fluorescent dyes, and identified a series of compounds in the xanthone family that exhibited consistent synaptic labeling. Follow-up studies with one of these compounds, CX-G3, demonstrated its ability to label acidic organelles and in particular synaptic vesicles at glutamatergic synapses in cultured neurons and murine brain tissue, indicating the potential of this screening approach to identify promising lead compounds for use as synaptic markers, sensors, and targeting devices.

Indexed as

NeuroimagingAnimalsCells, CulturedHippocampusNerve Tissue ProteinsNeuronsRats, Sprague-DawleySynapsesSynaptic VesiclesNerve Tissue Proteinsfluorescencehigh-content screeningimaging probeSynapsesynaptic vesiclexanthone

Identifiers

PMID29215865
PMCPMC8919986
OpenAlexW2771095710

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.