ArticleJournal of veterinary internal medicine2018
Neutrophil Extracellular Traps in Plasma from Dogs with Immune-mediated Hemolytic Anemia.
Article in Journal of veterinary internal medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- ClotPro viscoelastic testing in coagulation disorders in dogs: a retrospective analysis.Journal of veterinary internal medicine · 2026Article
- Non-anticoagulant heparin attenuates histone-mediated platelet-leukocyte aggregation and neutrophil extracellular trap formation in a canine whole blood model.Frontiers in veterinary science · 2026Article
- Tissue plasminogen activator modified thromboelastography identifies fibrinolysis resistance in dogs with immune-mediated hemolytic anemia.Frontiers in veterinary science · 2025Article
- Insight into antiphospholipid syndrome: the role and clinical utility of neutrophils extracellular traps formation.Thrombosis journal · 2024Article
- Preliminary Study on Treatment Outcomes and Prednisolone Tapering after Marine Lipid Extract EAB-277 Supplementation in Dogs with Immune-Mediated Hemolytic Anemia.Veterinary sciences · 2023Article
- Detection of Extracellular Traps in Canine Steroid-Responsive Meningitis-Arteritis.Frontiers in veterinary science · 2022Article
- Presumptive heterophil extracellular traps recognized cytologically in nine reptile patients with inflammatory conditions.The veterinary quarterly · 2021Article
- Methylprednisolone Induces Extracellular Trap Formation and Enhances Bactericidal Effect of Canine Neutrophils.International journal of molecular sciences · 2021Article
- Horizons in Veterinary Precision Oncology: Fundamentals of Cancer Genomics and Applications of Liquid Biopsy for the Detection, Characterization, and Management of Cancer in Dogs.Frontiers in veterinary science · 2021Review
- Neutrophil extracellular traps in stored canine red blood cell units.Journal of veterinary internal medicine · 2020Article
- Effect of sample type on plasma concentrations of cell-free DNA and nucleosomes in dogs.Veterinary record open · 2019Article
- Biomarker Guided Diagnosis of Septic Peritonitis in Dogs.Frontiers in veterinary science · 2019Article
- Bovine neutrophils form extracellular traps in response to the gastrointestinal parasite Ostertagia ostertagi.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNeutrophil extracellular traps (NETs) are part of the innate immune response and are essential in local pathogen control, but are associated with pathological inflammation, organ damage, autoimmunity, and thrombosis. Immune-mediated hemolytic anemia (IMHA) is a pro-inflammatory, prothrombotic disease associated with high mortality. HYPOTHESIS/
objectivesNeutrophil extracellular traps (NETs) are a feature of the inflammatory process in dogs with IMHA. The objective of the study was to evaluate plasma from dogs with IMHA for the presence of 2 indirect markers and 1 direct marker of NETs. ANIMALS: Healthy client-owned dogs (56) and hospitalized dogs with IMHA (n = 35).
methodsProspective study. Plasma samples for all dogs were evaluated for cell-free DNA using a fluorescence assay, histone-DNA (hisDNA) complex using an ELISA, and citrullinated histone H3 (specific for NETosis) using Western blot. Reference intervals were generated using plasma from healthy dogs.
resultsIn dogs with IMHA, cell-free DNA concentration was above the reference interval in 17% of samples with a median (range) of 1.0 μg/mL (0.1-17.3), and hisDNA concentration was above the reference interval in 94% of samples with a median (range) of 30.7 × pooled normal plasma (PNP; 0.6-372.1). Western blot for citrullinated histone H3 identified detectable bands in 84% samples from dogs with IMHA. CONCLUSIONS AND CLINICAL IMPORTANCE: The assay for cell-free DNA detected evidence of NETs in fewer dogs than did the other approaches. Excessive NETs appears to be a feature of IMHA in dogs and contributions to the prothrombotic state deserve further study.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.