ArticleBiotechnology for biofuels2017
Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release.
Article in Biotechnology for biofuels, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 119 citations in OpenAlex.
- Metabolic engineering for tobacco quality improvement: Advances and perspectives.Metabolic engineering communications · 2026Review
- Rhamnogalacturonan I is a recalcitrant pectin domain during Clostridium thermocellum-mediated deconstruction of switchgrass biomass.Biotechnology for biofuels and bioproducts · 2025Article
- 4-Coumarate CoA Ligase Family in Soybean Responds toCurrent issues in molecular biology · 2025Article
- Recent advances in breeding systems and their improvement in forage crops.Molecular biology reports · 2025Review
- Toward a monocot SynBio toolkit: assessing regulatory element performance and eudicot compatibility.Plant cell reports · 2025Article
- Advances in lignocellulosic feedstocks for bioenergy and bioproducts.Nature communications · 2025Review
- Cloning and functional analysis of Gb4CL1 and Gb4CL2 from Ginkgo biloba.The plant genome · 2024Article
- Blocking miR396 activity by overexpression MIM396 improved switchgrass tiller number and biomass yield.Biotechnology for biofuels and bioproducts · 2024Article
- Mutating alfalfaFrontiers in plant science · 2024Article
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
- Multi-allelic gene editing in an apomictic, tetraploid turf and forage grass (Frontiers in plant science · 2023Article
- Genome-edited rice deficient in two 4-COUMARATE:COENZYME A LIGASE genes displays diverse lignin alterations.Plant physiology · 2022Article
- Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.).Plant methods · 2022Article
- Recent advances in metabolic engineering of microorganisms for advancing lignocellulose-derived biofuels.Bioengineered · 2022Review
- Protoplast Isolation, Transformation, and Regeneration for Forage and Turf Grasses.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Spatio-Temporal Modification of Lignin Biosynthesis in Plants: A Promising Strategy for Lignocellulose Improvement and Lignin Valorization.Frontiers in bioengineering and biotechnology · 2022Review
- Genetic Determinants of Biomass in CFrontiers in plant science · 2022Review
- Gene Editing Technologies for Sugarcane Improvement: Opportunities and Limitations.Sugar tech : an international journal of sugar crops & related industries · 2022Article
- The Generic Risks and the Potential of SDN-1 Applications in Crop Plants.Plants (Basel, Switzerland) · 2021Review
- A gene-editing/complementation strategy for tissue-specific lignin reduction while preserving biomass yield.Biotechnology for biofuels · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe development of genome editing technologies offers new prospects in improving bioenergy crops like switchgrass (
resultsWe developed a CRISPR/Cas9 genome editing system in switchgrass to target a key enzyme involved in the early steps of monolignol biosynthesis, 4-Coumarate:coenzyme A ligase (4CL). Three
conclusionThis study established a successful CRISPR/Cas9 system in switchgrass with mutation efficiency reaching 10%. The system allows the precise targeting of the selected
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Registered trials
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