ArticleThe Journal of reproduction and development2018
Effects of insulin-like growth factor-1 on the in vitro maturation of canine oocytes.
Article in The Journal of reproduction and development, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Insulin-like growth factor 1 supplementation duringVeterinary world · 2026Article
- Insulin-like growth factor 1 as a key paracrine mediator in mesenchymal stem cell-based intervention for ovarian ageing: a review.Journal of ovarian research · 2026Review
- IGF-1 supplementation improvesFrontiers in veterinary science · 2026Article
- Effects of insulin-like growth factor-1 on mitogen-activated protein kinase and maturation promoting factor expressions, and mitochondrial DNA copy number in Kacang goat oocytes.Veterinary research forum : an international quarterly journal · 2026Article
- Advances in supplementation strategies for enhancing bovine in vitro embryo production: a review of growth factors and antioxidants.Journal of assisted reproduction and genetics · 2025Review
- Utilization of MSC-Derived Extracellular Vesicles and Bioscaffolds in Enhancing Oocyte In Vitro Maturation and Culture: A Review.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Potential of growth factors and steroid hormones from cell cultures to improveOpen veterinary journal · 2025Review
- Meiotic and developmental competence of growing pig oocytes derived from small antral follicles is enhanced in culture medium containing FGF2, LIF, and IGF1 (FLI medium).Journal of ovarian research · 2024Article
- Combination of CNP, MT and FLI during IVM Significantly Improved the Quality and Development Abilities of Bovine Oocytes and IVF-Derived Embryos.Antioxidants (Basel, Switzerland) · 2023Article
- Role of insulin-like growth factor 1 (IGF1) in the regulation of mitochondrial bioenergetics in zebrafish oocytes: lessons fromFrontiers in cell and developmental biology · 2023Article
- Insulin-like growth factor-1 improvesAnimal reproduction · 2023Article
- Simple and Efficient Chemically Defined In Vitro Maturation and Embryo Culture System for Bovine Embryos.Animals : an open access journal from MDPI · 2022Article
- Rescue IVM of Denuded GV- and MI-Stage Oocytes of Premenopausal Rats with Oncostatin M, Insulin-like Growth Factor I, and Growth Hormone.Life (Basel, Switzerland) · 2022Article
- Pleiotropic Effects of IGF1 on the Oocyte.Cells · 2022Article
- Growth hormone in fertility and infertility: Mechanisms of action and clinical applications.Frontiers in endocrinology · 2022Review
- The Role of MAPK3/1 and AKT in the Acquisition of High Meiotic and Developmental Competence of Porcine Oocytes Cultured In Vitro in FLI Medium.International journal of molecular sciences · 2021Article
- Fighting Like Cats and Dogs: Challenges in Domestic Carnivore Oocyte Development and Promises of Innovative Culture Systems.Animals : an open access journal from MDPI · 2021Review
- Beneficial Effects of L-Carnitine Supplementation during IVM of Canine Oocytes on Their Nuclear Maturation and Development In Vitro.Animals : an open access journal from MDPI · 2021Article
- "Immunolocalization and effect of low concentrations of Insulin like growth factor-1 (IGF-1) in the canine ovary".Veterinary medicine and science · 2021Article
- The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study.International journal of reproductive biomedicine · 2020Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The maturation rate of canine oocytes during in vitro maturation (IVM) needs to be improved. The present study was designed to evaluate the effects of insulin-like growth factor-1 (IGF-1) on the IVM of canine oocytes. Ovaries were obtained by ovariohysterectomy and were sliced to release cumulus-oocyte complexes (COCs). In Experiment 1, the effects of different concentrations of IGF-1 on the nuclear maturation of oocytes was investigated. The COCs were cultured in a modified medium (mTCM199) with IGF-1 (0, 0.5, 5, 10, and 50 µg/ml). At the end of the 48 h culture, oocytes were fixed and stained to evaluate their nuclear stage. Supplementation with 50 µg/ml IGF-1 induced a significantly higher metaphase II (MII) rate (P < 0.05) compared to the 0 and 0.5 μg/ml IGF-1 groups. In Experiment 2, the expression levels of insulin receptor (INSR), IGF-1 receptor (IGF-1R), and IGF-2 receptor (IGF-2R) genes, localized to canine oocytes and cumulus cells, were investigated before and after IVM. The expression level of IGF-1R in cumulus cells after IVM was higher than that before IVM (P < 0.05). In Experiment 3, it was investigated whether an inhibitor of PTEN (phosphatase and tensin homolog), bpV, affects the nuclear maturation of oocytes. Regardless of bpV supplementation at a concentration of 0.2 to 200 µmol/l, there was no significant difference in the proportion of oocytes that reached the MII stage. These results indicated that IGF-1 has a favorable effect on the IVM of canine oocytes, possibly through the stimulation of the Ras/MAPK pathway via IGF-1R expressed in cumulus cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.