ArticleDiagnostic pathology2017
Is hematoxylin-eosin staining in rectal mucosal and submucosal biopsies still useful for the diagnosis of Hirschsprung disease?
Article in Diagnostic pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 26 citations in OpenAlex.
- Automatic ganglion cell detection for improving the efficiency and accuracy of hirschprung disease diagnosis.Scientific reports · 2021Trial
- Clinicopathologic features of allied disorders of Hirschsprung disease and status update.World journal of gastrointestinal surgery · 2025Review
- Early Outcomes of Hirschsprung's Disease after Definitive Surgery: A Ten-year Experience.Current pediatric reviews · 2025Article
- Alterations in zinc, copper, and iron levels in the retina and brain of Alzheimer's disease patients and the APP/PS1 mouse model.Metallomics : integrated biometal science · 2024Article
- A new systematization of histological analysis for the diagnosis of Hirschsprung's disease.Clinics (Sao Paulo, Brazil) · 2023Article
- The utility of the 24-h delayed film of barium enema for detecting the dysganglionic bowel segment in Hirschsprung's disease.Frontiers in pediatrics · 2022Article
- Dietary supplementation withAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Article
- Global Proteomic Profile Integrated to Quantitative and Morphometric Assessment of Enteric Neurons: Investigation of the Mechanisms Involved in the Toxicity Induced by Acute Fluoride Exposure in the Duodenum.Neurotoxicity research · 2021Article
- Exploration of Potential Molecular Targets of Dexmedetomidine in the Intestinal Repair of Burnt Rats.Journal of inflammation research · 2021Article
- Protective Effect of Zuojin Fang on Lung Injury Induced by Sepsis through Downregulating the JAK1/STAT3 Signaling Pathway.BioMed research international · 2021Article
- In vitro and in vivo effects of 3-bromopyruvate against Echinococcus metacestodes.Veterinary research · 2019Article
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7 authors at 1 institution in 1 country.
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Abstract
backgroundHematoxylin-eosin (HE) staining of a full-thickness rectal wall fragment is classically used for the diagnosis of Hirschsprung disease (HD). However, this technique requires large fragments for a better diagnosis. Additionally, the histochemical and immunohistochemical methods of staining small fragments of rectal mucosal and submucosal biopsies are not available in all centers. Therefore, the possibility of diagnosing HD through HE staining in these biopsies could be a valuable alternative for centers that do not have more specific techniques. The objectives of the current investigation were to evaluate the concordance of the results obtained by HE staining and the calretinin method with acetylcholinesterase (AChE) activity in fragments of mucosa and submucosa in the diagnosis of HD.
methodsFor this study, 50 cases from our laboratory were selected. The tissue material was embedded in paraffin. Sixty levels of each fragment were utilized for HE, and the other 3 levels were used for calretinin. These slides were analyzed under the microscope, photographed and classified as either positive for HD when no ganglion cells were found with nerve trunks present or as negative when ganglion cells were found. The results from reading the slides were compared with those of AChE.
resultsOf the 50 cases evaluated by the HE technique, only 5 contradicted the diagnosis based on AChE, with a Kappa value of 0.800 and an accuracy of 90%. In the comparison between calretinin and AChE, 8 cases were discordant, with a Kappa value of 0.676 and an accuracy of 84%.
conclusionsThe concordance of results from AChE and HE methods was satisfactory, allowing for the potential use of the HE method for fragments of mucosa and submucosa as a valid alternative in the diagnosis of HD. The immunohistochemical technique of calretinin did not show good agreement with the AChE activity in our study.
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