ArticleBiophysical journal2017
Hallmarks of Reversible Separation of Living, Unperturbed Cell Membranes into Two Liquid Phases.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
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Who cites it
56 citing papers in PubMed, 95 citations in OpenAlex.
- Phase separation across membranes and condensates in cell organization and function.Nature reviews. Molecular cell biology · 2026Review
- The Lack of a COPII Cargo Receptor Erv14 Impacts Physiological Functions of the Vacuole in Saccharomyces cerevisiae.Traffic (Copenhagen, Denmark) · 2026Article
- Coupling between sterol and sphingolipid structure in ordered membrane domains.bioRxiv : the preprint server for biology · 2026Article
- ER exit sites mediated by the COPII adaptor sec24D selectively recruit lipid raft-preferring proteins for rapid ER export.Nature communications · 2025Article
- Septin higher-order structure on yeast membranes in vitro.Nature communications · 2025Article
- X-Ray Crystal and Cryo-Electron Microscopy Structure Analysis Unravels How the Unique Thylakoid Lipid Composition Is Utilized by CytochromeMembranes · 2025Article
- Structural dissection of ergosterol metabolism reveals a pathway optimized for membrane phase separation.Science advances · 2025Article
- Temperature dependence of membrane viscosity of ternary lipid GUV with LBiophysical journal · 2025Article
- Small-Molecule Modulators of Lipid Raft Stability and Protein-Raft Partitioning.bioRxiv : the preprint server for biology · 2024Article
- Sterol-lipids enable large-scale, liquid-liquid phase separation in bilayer membranes of only two components.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Coupling liquid phases in 3D condensates and 2D membranes: Successes, challenges, and tools.Biophysical journal · 2024Review
- Automated quantification of vacuole fusion and lipophagy inAutophagy · 2024Article
- G Protein-Coupled Receptor Dimerization-What Next?International journal of molecular sciences · 2024Review
- Diacylglycerol metabolism and homeostasis in fungal physiology.FEMS yeast research · 2024Review
- Intracellular sphingolipid sorting drives membrane phase separation in the yeast vacuole.The Journal of biological chemistry · 2024Article
- Using the yeast vacuole as a system to test the lipidic drivers of membrane heterogeneity in living cells.Methods in enzymology · 2024Article
- Physicochemical properties of the vacuolar membrane and cellular factors determine formation of vacuolar invaginations.Scientific reports · 2023Article
- Hybrid machine-learning framework for volumetric segmentation and quantification of vacuoles in individual yeast cells using holotomography.Biomedical optics express · 2023Article
- Lipid saturation controls nuclear envelope function.Nature cell biology · 2023Article
- Coupling of protein condensates to ordered lipid domains determines functional membrane organization.Science advances · 2023Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Controversy has long surrounded the question of whether spontaneous lateral demixing of membranes into coexisting liquid phases can organize proteins and lipids on micron scales within unperturbed, living cells. A clear answer hinges on observation of hallmarks of a reversible phase transition. Here, by directly imaging micron-scale membrane domains of yeast vacuoles both in vivo and cell free, we demonstrate that the domains arise through a phase separation mechanism. The domains are large, have smooth boundaries, and can merge quickly, consistent with fluid phases. Moreover, the domains disappear above a distinct miscibility transition temperature (T
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.