ArticlePloS one2017
Crystal structures of ternary complexes of archaeal B-family DNA polymerases.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Rapid evolution of a highly efficient RNA polymerase by homologous recombination.Nature chemical biology · 2026Article
- Archaeal family B DNA polymerase facilitates lagging strand DNA replication in the Thermococcales.Nucleic acids research · 2026Article
- Crystal structure of a closed ternary complex of a HNA Reverse Transcriptase in complex with a HNA/DNA duplex.PloS one · 2026Article
- Directed evolution of a TNA polymerase identifies independent paths to fidelity and catalysis.Nature communications · 2025Article
- Structural insights into a DNA polymerase reading the xeno nucleic acid HNA.Nucleic acids research · 2025Article
- Generation of densely labeled oligonucleotides for the detection of small genomic elements.Cell reports methods · 2024Article
- PPI3D: a web server for searching, analyzing and modeling protein-protein, protein-peptide and protein-nucleic acid interactions.Nucleic acids research · 2024Article
- Navigating directed evolution efficiently: optimizing selection conditions and selection output analysis.Frontiers in molecular biosciences · 2024Article
- Direct Enzyme Engineering of B Family DNA Polymerases for Biotechnological Approaches.Bioengineering (Basel, Switzerland) · 2023Review
- A two-residue nascent-strand steric gate controls synthesis of 2'-O-methyl- and 2'-O-(2-methoxyethyl)-RNA.Nature chemistry · 2023Article
- Introducing a New Bond-Forming Activity in an Archaeal DNA Polymerase by Structure-Guided Enzyme Redesign.ACS chemical biology · 2022Article
- Structural Basis for The Recognition of Deaminated Nucleobases by An Archaeal DNA Polymerase.Chembiochem : a European journal of chemical biology · 2021Article
- Developmental delay with hypotrophy associated with homozygous functionally relevant REV3L variant.Journal of molecular medicine (Berlin, Germany) · 2021Article
- Beyond the Lesion: Back to High Fidelity DNA Synthesis.Frontiers in molecular biosciences · 2021Review
- Structural Studies of HNA Substrate Specificity in Mutants of an Archaeal DNA Polymerase Obtained by Directed Evolution.Biomolecules · 2020Article
- Review
- How a B family DNA polymerase has been evolved to copy RNA.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Discovery and evolution of RNA and XNA reverse transcriptase function and fidelity.Nature chemistry · 2020Article
- Enzymatic Cleavage of 3'-Esterified Nucleotides Enables a Long, Continuous DNA Synthesis.Scientific reports · 2020Article
- An overview of 25 years of research on Thermococcus kodakarensis, a genetically versatile model organism for archaeal research.Folia microbiologica · 2020Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Archaeal B-family polymerases drive biotechnology by accepting a wide substrate range of chemically modified nucleotides. By now no structural data for archaeal B-family DNA polymerases in a closed, ternary complex are available, which would be the basis for developing next generation nucleotides. We present the ternary crystal structures of KOD and 9°N DNA polymerases complexed with DNA and the incoming dATP. The structures reveal a third metal ion in the active site, which was so far only observed for the eukaryotic B-family DNA polymerase δ and no other B-family DNA polymerase. The structures reveal a wide inner channel and numerous interactions with the template strand that provide space for modifications within the enzyme and may account for the high processivity, respectively. The crystal structures provide insights into the superiority over other DNA polymerases concerning the acceptance of modified nucleotides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.