ArticleMemorias do Instituto Oswaldo Cruz2017
Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers.
Article in Memorias do Instituto Oswaldo Cruz, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Interaction ofMicroorganisms · 2025Article
- Isolation and Molecular Identification of Pathogenic Free-Living Amoebae from Environmental Samples in Tenerife, Canary Islands, Spain.ACS ES&T water · 2025Article
- Effect of Cinnamaldehyde and Its Nanoemulsion on Acanthamoeba castellanii Cysts and Trophozoites.Acta parasitologica · 2025Article
- Detection of immunogenic protein components in excretion/secretion products of Acanthamoeba T5 using polyclonal antibodies.Memorias do Instituto Oswaldo Cruz · 2025Article
- Assessment ofJournal of water and health · 2024Article
- Overview of extracellular vesicles in pathogens with special focus on human extracellular protozoan parasites.Memorias do Instituto Oswaldo Cruz · 2024Review
- Plants of the family Lamiaceae as a source of therapeutic agents against Acanthamoeba infections.Memorias do Instituto Oswaldo Cruz · 2024Review
- Assessment of pathogenic potential of Acanthamoeba isolates by in vitro and in vivo tests.Parasitology research · 2023Article
- In Vitro Cytopathogenic Activities ofPathogens (Basel, Switzerland) · 2022Article
- Molecular detection and genotype identification ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Dual-Mode Gold Nanoparticle-Based Method for Early Detection ofInternational journal of molecular sciences · 2022Article
- Article
- High oxygen concentrations inhibit Acanthamoeba spp.Parasitology research · 2021Article
- Free-Living Amoebae in Soil Samples from Santiago Island, Cape Verde.Microorganisms · 2021Article
- Evaluation of the occurrence of pathogenic free-living amoeba and bacteria in 20 public indoor swimming pool facilities.MicrobiologyOpen · 2021Article
- Acanthamoeba species isolated from Philippine freshwater systems: epidemiological and molecular aspects.Parasitology research · 2020Article
- Microbial Contamination of Dental Unit Waterlines and Potential Risk of Infection: A Narrative Review.Pathogens (Basel, Switzerland) · 2020Review
- Identification of T3 and T4 Genotypes of Acanthamoeba sp. in Dust Samples Isolated from Air Conditioning Equipment of Public Hospital of Ituiutaba-MG.Current microbiology · 2020Article
- Combined Amoebicidal Effect of Atorvastatin and Commercial Eye Drops againstPathogens (Basel, Switzerland) · 2020Article
- Description of Virulent Factors and Horizontal Gene Transfers of Keratitis-Associated Amoeba Acanthamoeba Triangularis by Genome Analysis.Pathogens (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAcanthamoeba is the genus of free-living amoebae that is most frequently isolated in nature. To date, 20 Acanthamoeba genotypes have been described. Genotype T4 is responsible for approximately 90% of encephalitis and keratitis cases. Due to the ubiquitous presence of amoebae, isolation from environmental sources is not uncommon; to determine the clinical importance of an isolation, it is necessary to have evidence of the pathogenic potential of amoebae.
objectiveThe aim of this study was to physiologically characterise 8 Acanthamoeba T4 isolates obtained from dental units and emergency combination showers and to determine their pathogenic potential by employing different laboratory techniques.
methodsEight axenic cultures of Acanthamoeba genotype T4 were used in pathogenic potential assays. Osmotolerance, thermotolerance, determination and characterisation of extracellular proteases and evaluation of cytopathic effects in MDCK cells were performed.
findingsAll of the isolates were osmotolerant, thermotolerant and had serine proteases from 44-122 kDa. Two isolates had cytopathic effects on the MDCK cell monolayer. MAIN
conclusionThe presence of Acanthamoeba T4 with pathogenic potential in areas such as those tested in this study reaffirms the need for adequate cleaning and maintenance protocols to reduce the possibility of infection with free-living amoebae.
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